Relative expression of genes of menthol biosynthesis pathway in peppermint (Mentha piperita L.) after chitosan, gibberellic acid and methyl jasmonate treatments

Menthol as an important component of monoterpenes essential oil in peppermint ( Mentha piperita L.) is widely applied for medical and industrial uses. In this study, the effect of exogenous applications of chitosan (200 mg/L), gibberellic acid (50 mg/L) and methyl jasmonate (300 μM) was investigated...

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Bibliographic Details
Published in:Russian journal of plant physiology 2017, Vol.64 (1), p.59-66
Main Authors: Soleymani, F., Taheri, H., Shafeinia, A. R.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biosynthesis
Chitosan
Essential oils
Gene expression
Gene regulation
Genes
Gibberellic acid
Leaves
Life Sciences
Limonene
Mentha piperita
Menthol
Menthone
Methyl jasmonate
Monoterpenes
Peppermint oil
Plant Physiology
Plant Sciences
Plants (botany)
Polymerase chain reaction
Reductase
Research Papers
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Summary:Menthol as an important component of monoterpenes essential oil in peppermint ( Mentha piperita L.) is widely applied for medical and industrial uses. In this study, the effect of exogenous applications of chitosan (200 mg/L), gibberellic acid (50 mg/L) and methyl jasmonate (300 μM) was investigated in the main genes of menthol biosynthesis pathways within a 72 h time period using qRT-PCR. Transcript levels of most genes were either unaffected or down-regulated following chitosan treatment relative to control plants. Decreasing of geranyl diphosphate synthase ( GDS ) and limonene synthase ( LS ) genes transcript in chitosan treatment could possibly be effective in reducing of limonene level. On the other hand, it seems that an increase in menthone-menthol reductase ( MMR ) transcription level at 72 h under these treatments had a positive role in increasing the amount of menthol in this plant. Since exogenous application of gibberellic acid (GA 3 ) down-regulated transcript levels of several genes involved in menthol biosynthesis, there is this expectance that GA 3 treatment might not have a prominent role in enhancing menthol yield via transcription regulation. Transcript level of the majority genes after methyl jasmonate treatment gradually increased and reached the highest level at 72 h, therefore, it is possible that methyl jasmonate improves medicinal properties of M. piperita .
ISSN:1021-4437
1608-3407
DOI:10.1134/S1021443717010150