New insights for male infertility revealed by alterations in spermatic function and differential testicular expression of thyroid-related genes

The impact of thyroid hormone (TH) disorders on male reproductive biology has been a controversial issue for many years. Recently, we reported that hypothyroid male rats have a disruption of the seminiferous epithelium, which may compromise spermatogenesis. To improve the understanding of the reprod...

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Bibliographic Details
Published in:Endocrine 2017-02, Vol.55 (2), p.607-617
Main Authors: Romano, Renata Marino, Gomes, Samantha Nascimento, Cardoso, Nathalia Carolina Scandolara, Schiessl, Larissa, Romano, Marco Aurelio, Oliveira, Claudio Alvarenga
Format: Article
Language:English
Subjects:
Acrosome - physiology
Animals
Diabetes
Endocrinology
Epididymis
Epithelium
Gene expression
Humanities and Social Sciences
Hyperthyroidism
Hypothyroidism
Hypothyroidism - complications
Hypothyroidism - genetics
Hypothyroidism - physiopathology
Infertility
Infertility, Male - etiology
Infertility, Male - genetics
Infertility, Male - physiopathology
Internal Medicine
Iodide peroxidase
Iodide Peroxidase - genetics
Iodide Peroxidase - metabolism
Iodothyronine Deiodinase Type II
Male
Medicine
Medicine & Public Health
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Mitochondria
multidisciplinary
Original Article
Polymerase chain reaction
Rats
Rats, Wistar
Receptors, Thyroid Hormone - genetics
Receptors, Thyroid Hormone - metabolism
Reverse transcription
Rodents
Science
Sperm
Spermatogenesis
Spermatogenesis - physiology
Spermatozoa - physiology
Testes
Testis - metabolism
Thyroid
Thyroid gland
Thyroid Gland - physiopathology
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Summary:The impact of thyroid hormone (TH) disorders on male reproductive biology has been a controversial issue for many years. Recently, we reported that hypothyroid male rats have a disruption of the seminiferous epithelium, which may compromise spermatogenesis. To improve the understanding of the reproductive pathogenesis of hypothyroidism and hyperthyroidism, male Wistar rats that developed these dysfunctions in adulthood were used as an experimental model. We evaluated the sperm production, reserves, transit time, morphology, and functionality (acrosome integrity, plasma membrane integrity, and mitochondrial activity), and the testicular expression of the TH receptors ( Thra1 and Thra2 , Thrb1 , and Thrb2 ), deiodinases ( Dio2 and Dio3 ), and the Mct8 transporter ( Slc16a2 ) were assessed by reverse transcription followed by real-time quantitative PCR (RT-qPCR). The results were evaluated statistically by ANOVA and Tukey HSD test ( P  
ISSN:1355-008X
1559-0100
DOI:10.1007/s12020-016-0952-3