Resolving Co-Elution Problems of Components in Complex Mixtures by Multiple Heart-Cutting 2D-LC

Multiple heart-cutting (MHC) 2D-LC was applied to resolve challenging peak co-elutions in a pesticidal natural product-derived extract, which exhibited a complex impurity profile. While the 1D separation using sub-2 μm particle column technology provided good separation efficiency, additional select...

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Bibliographic Details
Published in:Chromatographia 2017-01, Vol.80 (1), p.31-38
Main Authors: Pursch, Matthias, Lewer, Paul, Buckenmaier, Stephan
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chromatography
Cutting
Laboratory Medicine
Natural products
Original
Pharmacy
Proteomics
Separation
Technology assessment
Two dimensional analysis
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Summary:Multiple heart-cutting (MHC) 2D-LC was applied to resolve challenging peak co-elutions in a pesticidal natural product-derived extract, which exhibited a complex impurity profile. While the 1D separation using sub-2 μm particle column technology provided good separation efficiency, additional selectivity was required to separate overlapping peaks in several chromatographic regions. MHC 2D-LC using C 18 columns in both dimensions, but different pH in the mobile phase, provided full separation of the components of interest. This article describes how the MHC 2D-LC technology has been applied for optimized analysis of target components as well as being a rapid tool for peak purity evaluation. It also discusses strategic benefits of the technology regarding method development for separation of very complex samples. Graphical abstract
ISSN:0009-5893
1612-1112
DOI:10.1007/s10337-016-3214-x