Bio-deuterated cellulose thin films for enhanced contrast in neutron reflectometry

• Published in: Cellulose (London) 2017, Vol.24 (1), p.11-20
• Main Authors: Raghuwanshi, Vikram Singh, Su, Jielong, Garvey, Christopher J., Holt, Stephen A., Raverty, Warwick, Tabor, Rico F., Holden, Peter J., Gillon, Marie, Batchelor, Warren, Garnier, Gil
• Format: Article
• Language: English
• Subjects: Biomolecules; Bioorganic Chemistry; Biosynthesis; Cellulose; Ceramics; Chemical bonds; Chemistry; Chemistry and Materials Science; Composites; Deuteration; Glass; Hydroxyl groups; Liquid phases; Macromolecules; Natural Materials; Organic Chemistry; Original Paper; Physical Chemistry; Polymer Sciences; Sustainable Development; Thin films
• ISSN: 0969-0239; 1572-882X
• DOI: 10.1007/s10570-016-1108-6

Novel molecularly smooth, flat and thin films of regenerated bio-deuterated cellulose were produced for enhanced contrast with adsorbed molecules in neutron reflectivity (NR) and for cellulose structure studies. The cellulose films were produced to study both the solid/air interface and the solid/liquid interface. Cellulose films with a wide range of scattering contrast were achieved by combining exchange of 1 H for deuterium on hydroxyl groups via water in the liquid phase and via biosynthesis of deuterated bacterial cellulose by Gluconacetobacter xylinus which can deuterate the hydrogens bonded to carbon atoms in cellulose. The deuterated cellulose combined with NR will help to provide new information on the interaction of various (bio)-macromolecules and cellulose. This includes quantifying and visualizing the density profile of polymers and biomolecules adsorbed onto cellulose surface. The potential of this material for IR studies of materials adsorbed to cellulose films is briefly discussed.