Production of Cellulases by Phanerochaete sp. Using Empty Fruit Bunches of Palm (EFB) as Substrate: Optimization and Scale-Up of Process in Bubble Column and Stirred Tank Bioreactors (STR)

The goal of this study was to screen microorganisms that are potential producers of cellulases, determine optimal conditions for production of these enzymes, and scale up the process, using empty fruit bunches (EFB) as a substrate. Strains of Trichoderma sp., Aspergillus niger , Phanerochaete sp., G...

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Bibliographic Details
Published in:Waste and biomass valorization 2016-12, Vol.7 (6), p.1327-1337
Main Authors: Maceno, Marcelo Adriano Corrêa, de Souza Vandenberghe, Luciana Porto, Woiciechowski, Adenise Lorenci, Soccol, Carlos Ricardo, Spier, Michele Rigon
Format: Article
Language:English
Subjects:
Biomass
Bioreactors
Bubble columns
Cellulase
Crystalline cellulose
Engineering
Environment
Environmental Engineering/Biotechnology
Enzymes
Fermentation
Industrial Pollution Prevention
Microorganisms
Optimization
Original Paper
Potassium phosphates
Renewable and Green Energy
Solid suspensions
Substrates
Waste Management/Waste Technology
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Summary:The goal of this study was to screen microorganisms that are potential producers of cellulases, determine optimal conditions for production of these enzymes, and scale up the process, using empty fruit bunches (EFB) as a substrate. Strains of Trichoderma sp., Aspergillus niger , Phanerochaete sp., Ganoderma sp. and Lentinus sp. were evaluated for their ability to produce cellulolytic enzyme complexes under submerged with suspended solids fermentation (SSdF). Among these microorganisms, Phanerochaete sp. (PH-HD), which is already known to be a good producer of lignolytic enzymes, presented the highest capacity for cellulase production. The highest level of cellulase production by Phanerochaete sp. was observed on the 4th day of fermentation at 28 °C and pH 5.7, reaching a value of FPase activity of 364 IU L −1 using 15 g L −1 of EFB as a carbon source, 2 g L −1 of urea as a nitrogen source, 4 g L −1 of KH 2 PO 4 , and microcrystalline cellulose Avicel ® PH-101 as an inducer (2 g L −1 ). This study shows the great potential of Phanerochaete sp. for producing cellulolytic enzymes, as well as the feasibility of using EFB under suspended solids fermentation in the production process.
ISSN:1877-2641
1877-265X
DOI:10.1007/s12649-016-9503-7