The Use of the PHOTOCHEM Device in Evaluation of Antioxidant Activity of Polish Honey

Forty samples of varietal Polish honeys were investigated in order to assess their antioxidant capacity by photochemiluminescence (PCL) methods using standard ACW and ACL kits. The reference methods, FRAP and DPPH, were used. The total phenolics content (TPC) by Folin-Ciocalteau method was determine...

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Bibliographic Details
Published in:Food analytical methods 2017-05, Vol.10 (5), p.1568-1574
Main Authors: Wesołowska, Monika, Dżugan, Małgorzata
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antioxidants
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Correlation analysis
Food Science
Honey
Lipids
Microbiology
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Summary:Forty samples of varietal Polish honeys were investigated in order to assess their antioxidant capacity by photochemiluminescence (PCL) methods using standard ACW and ACL kits. The reference methods, FRAP and DPPH, were used. The total phenolics content (TPC) by Folin-Ciocalteau method was determined. The antioxidant properties of honeys as well as TPC were mainly dependent on honey botanical origin. The highest phenolic content and antioxidant activity for dark honeys, while the lowest for white honeys, were found. Moreover, the results obtained by used analytical methods were comparable and the best correlation between PCL and FRAP ( r  > 0.81) and DPPH ( r  = 0.74) methods was observed. The TPC in honeys was positively correlated with its antioxidant activity measured by PCL ( r  > 0.76). Used for the first time, PCL assay demonstrated usefulness in determining the antioxidant capacity of honey, divided into water-and lipid-soluble fractions, measured by ACW and ACL kits, respectively.
ISSN:1936-9751
1936-976X
DOI:10.1007/s12161-016-0715-z