Optimization of Enzyme-assisted Extraction of Antitumor Polysaccharides from Hericium erinaceus mycelia

In this study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from Hericium erinaceus mycelia (HEP) using the Box-Behnken design based on the results of single factor experiments. As a result, the optimal protocol was as follows: an...

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Bibliographic Details
Published in:FOOD SCIENCE AND TECHNOLOGY RESEARCH 2017, Vol.23(1), pp.31-39
Main Authors: Qin, Yi, Zhang, Zuofa, Song, Tingting, Lv, Guoying
Format: Article
Language:English
Subjects:
antitumor activity
Arabinose
Drugs
enzyme-assisted extraction
Enzymes
Extraction
Fucose
Galactose
Hericium erinaceus mycelia
Mannose
Mycelia
Optimization
pH effects
Polysaccharides
Raw materials
Saccharides
Temperature effects
Xylose
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Summary:In this study, an efficient complex enzyme-assisted extraction technology was developed and optimized to extract polysaccharides from Hericium erinaceus mycelia (HEP) using the Box-Behnken design based on the results of single factor experiments. As a result, the optimal protocol was as follows: an extraction time of 79 min, an extraction temperature of 50°C, a pH of 5.7 and a ratio of water to raw materials of 33.4 mL/g. These optimal extraction conditions achieved the highest yield (13.9 ± 0.3%) of HEP. The purified fraction (HEP-2) was composed of glucose, galactose, mannose, fucose, arabinose, xylose and rhammnose. HEP-2 manifested an obvious inhibitory effect on the growth of HeLa cells. The overall findings implied that enzyme-assisted procedure is a superior method and that HEP-2 might be useful for developing natural and safe antitumor drugs.
ISSN:1344-6606
1881-3984
DOI:10.3136/fstr.23.31