Role of bacterial pyrroloquinoline quinone in phosphate solubilizing ability and in plant growth promotion on strain Serratia sp. S119

The aim of this study was to analyze if cofactor pyrroquinoline quinone from Serratia sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqq E- minus mutant of strain Serratia sp...

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Bibliographic Details
Published in:Symbiosis (Philadelphia, Pa.) Pa.), 2017-05, Vol.72 (1), p.31-43
Main Authors: Ludueña, Liliana Mercedes, Anzuay, María Soledad, Angelini, Jorge Guillermo, Barros, Germán, Luna, María Flavia, Monge, María del Pilar, Fabra, Adriana, Taurian, Tania
Format: Article
Language:English
Subjects:
Bans
Biomedical and Life Sciences
Complementation
Conserved sequence
Developmental Biology
Ecology
Evolutionary Biology
Gluconic acid
Inoculation
Life Sciences
Microbiology
Plant growth
Plant Sciences
Pyrroloquinoline quinone
Quinones
Site-directed mutagenesis
Solubilization
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Summary:The aim of this study was to analyze if cofactor pyrroquinoline quinone from Serratia sp. S119 is involved in the inorganic phosphate solubilization mechanism and in its ability to promote the plant growth. Site directed mutagenesis was performed to obtain a pqq E- minus mutant of strain Serratia sp. S119. The phosphate solubilization ability, gluconate and PQQ production of the mutant Serratia sp. RSL ( pqq E-) was analyzed. Mutant RSL ( pqq E-) showed significant decrease in P soluble and gluconic acid levels produced and undetectable levels of PQQ cofactor compared with wild-type strain. Complementation with synthetic PQQ cofactor restored P solubilization and gluconate production reaching the levels produced by wild-type strain. PqqE gene sequence indicated that it is highly conserved within Serratia strains and its product shows conserved motifs found in other PqqE proteins of several bacteria. The effect of the inoculation of the PQQ- mutant on peanut and maize plants was evaluated in pot assays. Plants growth parameters showed no differences among the different treatments indicating that PQQ from Serratia sp. S119 is not involved in the growth promotion of these plants. PQQ cofactor is essential for phosphate solubilization ability of Serratia sp. S119 but is not required for growth promotion of peanut and maize plants.
ISSN:0334-5114
1878-7665
DOI:10.1007/s13199-016-0434-7