The comparison of the Wnt signaling pathway inhibitor delivered electrospun nanoyarn fabricated with two methods for the application of urethroplasty

Urethral strictures were common disease caused by over-expression of extracellular matrix from fibroblast. In this study, we compare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the over-expression of extracellular matrix. Collagenlpoly（L-lactide-co-caprolactone）（...

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Bibliographic Details
Published in:Frontiers of materials science 2016-12, Vol.10 (4), p.346-357
Main Authors: GUO, Xuran, ZHANG, Kaile, EL-AASSAR, Mohamed, WANG, Nanping, EL-HAMSHARY, Hany, EL-NEWEHY, Mohamed, FU, Qiang, MO, Xiumei
Format: Article
Language:English
Subjects:
biomaterials
Chemistry and Materials Science
Collagen
Electrospinning
Extracellular matrix
Fibroblasts
Infiltration
inhibitor
Inhibitors
Kinases
Materials Science
Mechanical properties
Morphology
nanoyarn
Research Article
Scaffolds
SEM观察
Signaling
Wnt信号通路
制备
尿道狭窄
成纤维细胞
抑制剂
细胞外基质
静电纺丝法
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Summary:Urethral strictures were common disease caused by over-expression of extracellular matrix from fibroblast. In this study, we compare two nanoyarn scaffolds for improving fibroblasts infiltration without inhibition the over-expression of extracellular matrix. Collagenlpoly（L-lactide-co-caprolactone）（ColIP（LLA-CL）） nanoyarn scaffolds were prepared by conjugated electrospinning and dynamic liquid electrospinning, respectively, in addition, co-axial electrospinning technique was combined with the nanoyarn fabrication process to produce nanoyarn scaffolds loading Wntsignaling pathway inhibitor. The mechanical properties of the scaffolds were examined and morphology was observed by SEM. Cell morphology, proliferation and infiltration on the scaffolds were investigated by SEM, MTT assay and H＆E staining, respectively. The release profiles of different scaffolds were determined using HPLC. The results indicated that cells showed an organized morphology along the nanoyarns and considerable infiltration into the nanoyarn scaffolds prepared by dynamic liquid electrospinning （DLY）. It was also observed that the DLY significantly facilitate cell proliferation. The D-DLY could facilitate the infiltration of the fibroblasts and could be a promising scaffold for the treatment of urethra stricture while it may inhibit the collagen production.
ISSN:2095-025X
2095-0268
DOI:10.1007/s11706-016-0359-3