Block Copolymer Micellization as a Protection Strategy for DNA Origami

DNA nanotechnology enables the synthesis of nanometer‐sized objects that can be site‐specifically functionalized with a large variety of materials. For these reasons, DNA‐based devices such as DNA origami are being considered for applications in molecular biology and nanomedicine. However, many DNA...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2017-05, Vol.56 (20), p.5460-5464
Main Authors: Agarwal, Nayan P., Matthies, Michael, Gür, Fatih N., Osada, Kensuke, Schmidt, Thorsten L.
Format: Article
Language:English
Subjects:
Biology
Block copolymers
Body fluids
Cell culture
Copolymers
Deoxyribonucleic acid
DNA
DNA - chemistry
DNA biosynthesis
DNA nanotechnology
DNA origami
Ionic strength
Micelles
Molecular biology
Molecular Structure
nanobiomedicine
Nanostructures - chemistry
Nanotechnology
Nuclease
Particle Size
Polyethylene glycol
Polyethylene Glycols - chemistry
Polylysine
Polylysine - chemistry
polyplex micelles
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Summary:DNA nanotechnology enables the synthesis of nanometer‐sized objects that can be site‐specifically functionalized with a large variety of materials. For these reasons, DNA‐based devices such as DNA origami are being considered for applications in molecular biology and nanomedicine. However, many DNA structures need a higher ionic strength than that of common cell culture buffers or bodily fluids to maintain their integrity and can be degraded quickly by nucleases. To overcome these deficiencies, we coated several different DNA origami structures with a cationic poly(ethylene glycol)–polylysine block copolymer, which electrostatically covered the DNA nanostructures to form DNA origami polyplex micelles (DOPMs). This straightforward, cost‐effective, and robust route to protect DNA‐based structures could therefore enable applications in biology and nanomedicine where unprotected DNA origami would be degraded. Polymeric protection: DNA origami nanostructures generally need higher ionic strengths than those of common cell culture buffers to maintain their integrity and are quickly degraded by nucleases. They can, however, be protected from these processes through the formation of polyplex micelles with poly(ethylene glycol)‐b‐polylysine.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201608873