RELEASE OF ENDOGENOUS GLUTAMATE FROM THE FROG SPINAL CORD FOLLOWING DORSAL ROOT STIMULATION

The effects of dorsal root stimulation on the release of endogenous glutamate and aspartate from the hemisected or intra-arterially perfused frog spinal cord were studied. The release of amino acids was measured using a gas chromatograph-mass spectrometer. Repetitive stimulation applied to the dorsa...

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Bibliographic Details
Published in:Biomedical Research 1985/08/01, Vol.6(4), pp.239-245
Main Authors: KAWAGOE, REIKO, ONODERA, KAYOKO, TAKEUCHI, AKIRA
Format: Article
Language:English
Subjects:
Amino acids
Dorsal roots
Efflux
Gas chromatography
Spinal cord
Stimulation
Synaptic transmission
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Summary:The effects of dorsal root stimulation on the release of endogenous glutamate and aspartate from the hemisected or intra-arterially perfused frog spinal cord were studied. The release of amino acids was measured using a gas chromatograph-mass spectrometer. Repetitive stimulation applied to the dorsal roots produced ventral root potentials and caused a significant increase in the glutamate releases above the resting efflux. In low Ca solution, the ventral root potential was abolished and the glutamate efflux following dorsal root stimulation was significantly decreased. In these experiments, the stimulus strength was adjusted just maximal for the fast ventral root potential. The resting efflux and the evoked release of aspartate caused by dorsal root stimulation was much smaller than the glutamate release. It is suggested that glutamate is an excitatory transmitter in primary afferents that mediates the fast synaptic transmission in the frog spinal cord.
ISSN:0388-6107
1880-313X
DOI:10.2220/biomedres.6.239