High-throughput sequencing methods (RNA sequencing and Poly(A)-sequencing) to characterize full-length messenger RNA in transcriptionally silent bull sperm; Potential noninvasive measures of bull fertility

• Published in: Journal of animal science 2017-03, Vol.95, p.151
• Main Author: Sartini, B L
• Format: Article
• Language: English
• Subjects: Biological activity; Fertility; Gene sequencing; mRNA; Next-generation sequencing; Poly(A); Populations; Protein kinase; Ribonucleic acid; RNA; Sperm; Transcription
• ISSN: 0021-8812; 1525-3163
• DOI: 10.2527/asasmw.2017.310

An abstract of a study by Sartini comparing the oligo-dT selected spermatozoal transcript profiles of higher fertility (conception rate [CR] 1.8 to 3.5) and lower fertility (CR -2.9 to -0.4) sires using RNA-sequencing is presented. A total of 3,227 and 5,366 transcripts were identified in the higher and lower fertility populations, respectively. Although transcripts common between the 2 populations were identified (2,422 transcripts), several transcripts were also unique to the fertility populations, including 805 transcripts that were unique to the higher fertility population and 2,944 transcripts that were unique to the lower fertility population. From gene ontological analysis, the transcripts unique to each fertility population differed in Biological Processes, including enrichment of regulatory transcripts for growth and protein kinase activity in the higher-fertility bulls. Biological variation in transcript presence among individual sires was also found, and transcripts with a correlation to sire fertility were identified.