Identification of alternatively spliced transcripts of rice phytochelatin synthase 2 gene OsPCS2 involved in mitigation of cadmium and arsenic stresses

• Published in: Plant molecular biology 2017-05, Vol.94 (1-2), p.167-183
• Main Authors: Das, Natasha, Bhattacharya, Surajit, Bhattacharyya, Somnath, Maiti, Mrinal K.
• Format: Article
• Language: English
• Subjects: Alternative splicing; Alternative Splicing - drug effects; Amino Acid Sequence; Aminoacyltransferases - genetics; Aminoacyltransferases - metabolism; Arsenic; Arsenic - metabolism; Arsenic - toxicity; Biochemistry; Biomedical and Life Sciences; Cadmium; Cadmium - metabolism; Cadmium - toxicity; Cloning, Molecular; Cultivars; DNA, Plant; Endosperm; Enzymatic activity; Gene Expression Regulation, Plant - physiology; Gene regulation; Gene silencing; Genes; Genome, Plant; Heavy metals; Life Sciences; Nonsense mutation; Oryza - drug effects; Oryza - genetics; Oryza - metabolism; Oryza sativa; Phytochelatin synthase; Phytochelatins; Plant Pathology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Roots - genetics; Plant Roots - metabolism; Plant Sciences; Plant Shoots - genetics; Plant Shoots - metabolism; Promoter Regions, Genetic; Relative abundance; Ribonucleic acid; Rice; RNA; RNA-mediated interference; Seeds; Stress analysis; Stress, Physiological - drug effects; Toxicity; Transcription; Transgenic plants; Yeasts
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/s11103-017-0600-1

Key message The OsPCS2 exhibits root- and shoot-specific differential ratios of alternatively spliced transcripts in indica rice under Cd stress, and plays role in Cd and As stress tolerance and accumulation. Enzymatic activity of phytochelatin synthase (PCS) in plant produces phytochelatins, which help in sequestration of heavy metal(loid)s inside the cell vacuole to alleviate toxicity. Here we report that among the two PCS genes— OsPCS1 and OsPCS2 in indica rice ( Oryza sativa ) cultivar, the OsPCS2 produces an alternatively spliced OsPCS2b transcript that bears the unusual premature termination codon besides the canonically spliced OsPCS2a transcript. Root- and shoot-specific differential ratios of alternatively spliced OsPCS2a and OsPCS2b transcript expressions were observed under cadmium stress. Saccharomyces cerevisiae cells transformed with OsPCS2a exhibited increased cadmium (Cd) and arsenic (As) tolerance and accumulation, unlike the OsPCS2b transformed yeast cells. An intron-containing hairpin RNA-mediated gene silencing was carried out in endosperm-specific manner for efficient down-regulation of OsPCS genes in rice grains. Analysis of the transgenic rice lines grown under metal(loid) stress revealed almost complete absence of both OsPCS1 and OsPCS2 transcripts in the developing seeds coupled with the significant reduction in the content of Cd (~51%) and As (~35%) in grains compared with the non-transgenic plant. Taken together, the findings indicate towards a crucial role played by the tissue-specific alternative splicing and relative abundance of the OsPCS2 gene during heavy metal(loid) stress mitigation in rice plant.