Enhanced Production of Nitrilase from Streptomyces sp. MTCC 7546 by Response Surface Method

• Published in: Proceedings of the National Academy of Sciences, India. Section B: Biological sciences India. Section B: Biological sciences, 2017-06, Vol.87 (2), p.603-609
• Main Authors: Agarwal, Astha, Nigam, Vinod Kumar
• Format: Article
• Language: English
• Subjects: Behavioral Sciences; Benzonitrile; Biomedical and Life Sciences; Bioremediation; Carbon sources; Enzymes; Glucose; Life Sciences; Nitrilase; Nucleic Acid Chemistry; Plant Biochemistry; Research Article
• ISSN: 0369-8211; 2250-1746
• DOI: 10.1007/s40011-015-0638-2

Nitrilases are enzymes with varied applications preferably used in preparation of industrially important compounds as well as in bioremediation processes. The production of enzymes is influenced by medium compositions as some of the constituents of medium directly affect the enzyme synthesis. In the present study, the components of the medium are optimized by classical and response surface methodology to maximize the production of intracellular nitrilase from Streptomyces sp. MTCC 7546. Glucose was observed as the most suitable carbon source for production of nitrilase by ‘one factor at a time’. The optimal concentration of glucose (5 g/L) was set as zero coded level for further optimization study. A central composite design was employed to study the effect of interaction among glucose, yeast extract and mineral base and evaluated for maximum nitrilase yield. The nitrilase production of 11.54 U/mg was observed at 24 h in medium containing 6.5 g/L glucose, 1.0 g/L yeast extract and 220 mL/L mineral base with 10 mM of benzonitrile as inducer. A twofold increment from 5.95 U/mg in the enzyme yield was observed after the optimization study of response surface methodology.