Near‐Infrared Photothermally Activated DNAzyme–Gold Nanoshells for Imaging Metal Ions in Living Cells

DNAzymes have enjoyed success as metal ion sensors outside cells. Their susceptibility to metal‐dependent cleavage during delivery into cells has limited their intracellular applications. To overcome this limitation, a near‐infrared (NIR) photothermal activation method is presented for controlling D...

Full description

Saved in:
Bibliographic Details
Published in:Angewandte Chemie International Edition 2017-06, Vol.56 (24), p.6798-6802
Main Authors: Wang, Wenjing, Satyavolu, Nitya Sai Reddy, Wu, Zhenkun, Zhang, Jian‐Rong, Zhu, Jun‐Jie, Lu, Yi
Format: Article
Language:English
Subjects:
Cells (biology)
Cleavage
DNA, Catalytic - metabolism
DNAzymes
Electrophoresis, Polyacrylamide Gel
Enzyme Activation
Gold
Gold - chemistry
gold nanoshells
HeLa Cells
Humans
Hybridization
I.R. radiation
Illumination
Infrared Rays
Metal ions
Nanoshells - chemistry
Near infrared radiation
photothermal activation
Phototoxicity
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet
Temperature
Temperature effects
Zinc
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNAzymes have enjoyed success as metal ion sensors outside cells. Their susceptibility to metal‐dependent cleavage during delivery into cells has limited their intracellular applications. To overcome this limitation, a near‐infrared (NIR) photothermal activation method is presented for controlling DNAzyme activity in living cells. The system consists of a three‐stranded DNAzyme precursor (TSDP), the hybridization of which prevents the DNAzyme from being active. After conjugating the TSDP onto gold nanoshells and upon NIR illumination, the increased temperature dehybridizes the TSDP to release the active DNAzyme, which then carries out metal‐ion‐dependent cleavage, resulting in releasing the cleaved product containing a fluorophore. Using this construct, detecting Zn2+ in living HeLa cells is demonstrated. This method has expanded the DNAzyme versatility for detecting metal ions in biological systems under NIR light that exhibits lower phototoxicity and higher tissue penetration ability. Gold in the shell: A near‐IR light‐activated DNAzyme–gold nanoshell system has been developed. It was then applied for imaging metal ions in living cells.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.201701325