Fc[epsilon]RI cross-linking reduces cord blood dendritic cell responsiveness to LPS

• Published in: Journal of allergy and clinical immunology 2017-06, Vol.139 (6), p.1992
• Main Authors: Paveglio, Sara, Bennett, Erin, Hawley, Kelly L, Matson, Adam P
• Format: Article
• Language: English
• Subjects: Allergies; Antigens; Cord blood; Cytokines; Dendritic cells; Immune response; Innate immunity; Ligands; Lipopolysaccharides; Neutrophils; Rodents; Studies
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2016.11.012

Given their ability to guide both tolerogenic and inflammatory responses, DCs may be especially relevant to immune programming in early life. [...]we evaluated the effects of Fc[epsilon]RI stimulation on CB myeloid DCs (mDCs). [...]anti-Fc[epsilon]RI stimulation of purified CB mDCs demonstrated only a modest decrease in LPS-induced production of proinflammatory cytokines (Fig 1, G), suggesting that the suppressive effects are enhanced when Fc[epsilon]RI activation occurs within a mixed population of cells. Because Fc[epsilon]RI stimulation had a greater impact on DC responsiveness in a mixed population of cells, we reasoned that a soluble factor produced by another Fc[epsilon]RI-expressing cell type, such as basophils, might contribute to the inhibitory effect. [...]mixed CB leukocytes were treated with LPS and/or physiologic doses of histamine and analyzed to quantify intracellular TNF-α production by CB mDCs. [...]our observation that Fc[epsilon]RI stimulation diminishes CB mDC responsiveness to LPS supports a growing body of evidence that cross-talk between IgE/Fc[epsilon]RI and TLR signaling pathways serves a regulatory purpose.2,6,7 Importantly, we reveal a novel pathway for the regulation of DC-driven innate immune responses in the newborn that can be mediated by Fc[epsilon]RI cross-linking and secretory products such as histamine.