Dynamic spatio-temporal contribution of single [beta]5t+ cortical epithelial precursors to the thymus medulla

Intrathymic T-cell development is critically dependent on cortical and medullary thymic epithelial cells (TECs). Both epithelial subsets originate during early thymus organogenesis from progenitor cells that express the thymoproteasome subunit [beta]5t, a typical feature of cortical TECs. Using in v...

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Bibliographic Details
Published in:European journal of immunology 2016-04, Vol.46 (4), p.846
Main Authors: Mayer, Carlos E, uklys, Saulius, Zhanybekova, Saule, Ohigashi, Izumi, Teh, Hong-Ying, Sansom, Stephen N, Shikama-Dorn, Noriko, Hafen, Katrin, Macaulay, Iain C, Deadman, Mary E, Ponting, Chris P, Takahama, Yousuke, Hollander, Georg A
Format: Article
Language:English
Subjects:
Cell fate
Epithelial cells
Fate maps
Lymphocytes T
Medulla oblongata
Mice
Organogenesis
Rodents
Stem cells
Temporal lobe
Thymus
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Summary:Intrathymic T-cell development is critically dependent on cortical and medullary thymic epithelial cells (TECs). Both epithelial subsets originate during early thymus organogenesis from progenitor cells that express the thymoproteasome subunit [beta]5t, a typical feature of cortical TECs. Using in vivo lineage fate mapping, we demonstrate in mice that [beta]5t+ TEC progenitors give rise to the medullary TEC compartment early in life but significantly limit their contribution once the medulla has completely formed. Lineage-tracing studies at single cell resolution demonstrate for young mice that the postnatal medulla is expanded from individual [beta]5t+ cortical progenitors located at the cortico-medullary junction. These results therefore not only define a developmental window during which the expansion of medulla is efficiently enabled by progenitors resident in the thymic cortex, but also reveal the spatio-temporal dynamics that control the growth of the thymic medulla.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.201545995