Applicability of TOF-SIMS for the assessment of lipid composition of cell membrane structures

Langmuir–Blodgett lipid films and plasma membranes of glioma cells were analyzed using timeof-flight secondary ion mass spectrometry (TOF-SIMS). Bi 3 + primary ion beam was determined to be most efficient in various experimental setups. TOF-SIMS was shown to be applicable for a quantitative analysis...

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Bibliographic Details
Published in:Biochemistry (Moscow). Supplement series A, Membrane and cell biology Membrane and cell biology, 2017-04, Vol.11 (2), p.144-150
Main Authors: Gulin, A. A., Pavlyukov, M. S., Gusev, S. A., Malakhova, Yu. N., Buzin, A. I., Chvalun, S. N., Aldarov, K. G., Klinov, D. V., Gularyan, S. K., Nadtochenko, V. A.
Format: Article
Language:English
Subjects:
Atomic force microscopy
Biomedical and Life Sciences
Brain tumors
Cell Biology
Cell surface
Cellular biology
Electron microscopy
Glioma
Glioma cells
In vitro methods and tests
Langmuir-Blodgett films
Life Sciences
Lipid composition
Lipids
Mass spectrometry
Mass spectroscopy
Membrane structures
Membranes
Plasma membranes
Quantitative analysis
Scanning electron microscopy
Secondary ion mass spectrometry
Water analysis
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Summary:Langmuir–Blodgett lipid films and plasma membranes of glioma cells were analyzed using timeof-flight secondary ion mass spectrometry (TOF-SIMS). Bi 3 + primary ion beam was determined to be most efficient in various experimental setups. TOF-SIMS was shown to be applicable for a quantitative analysis of model lipid structures, as well as plasma membranes of glioma cells U87MG in vitro. A combination of atomic-force microscopy and scanning electron microscopy yielded the depth resolution of ~10–20 nm for cell surface scanning by primary ion beam.
ISSN:1990-7478
1990-7494
DOI:10.1134/S1990747817020052