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Drug resistance mutations and susceptibility phenotypes of Neisseria gonorrhoeae isolates in Russia
Steady growth in the degree of antimicrobial resistance in Neisseria gonorrhoeae calls for the control of the spreading of resistance mutations. Here we present the data describing drug resistance mutations, the results of antimicrobial susceptibility tests, and molecular genotypes of 128 recent N....
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Published in: | Molecular biology (New York) 2017-05, Vol.51 (3), p.379-388 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Steady growth in the degree of antimicrobial resistance in
Neisseria gonorrhoeae
calls for the control of the spreading of resistance mutations. Here we present the data describing drug resistance mutations, the results of antimicrobial susceptibility tests, and molecular genotypes of 128 recent
N. gonorrhoeae
isolates collected across 9 regions of the Russian Federation. The mutations in chromosome genes
penA
,
ponA
,
rpsJ
,
gyrA
,
parC
, which determine the susceptibility of
N. gonorrhoeae
to penicillins, tetracyclines, and fluoroquinolones were detected by multiplex amplification followed by hybridization on a hydrogel microarray. The most frequent mutation was an insertion of an aspartate at position 345 of
penA
gene (76.6%), whereas mutations Leu421Pro in
ponA
gene, Val57Met in
rpsJ
gene, Ser91Phe in
gyrA
gene, Asp95Gly in
gyrA
gene, and Ser87Arg in
parC
gene were detected in 32.8–36.7% of strains. One third of studied
N. gonorrhoeae
isolates harbored multiple drug resistance mutations in bacterial chromosome, resulting in the bimodal distribution of mutation profiles and related patterns of antimicrobial susceptibility. The spread of multiple resistance could be explained by the vertical transfer of the mutations resulting in the clonality of the
N. gonorrhoeae
population. |
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ISSN: | 0026-8933 1608-3245 |
DOI: | 10.1134/S0026893317030116 |