Chemiliminescence determination of lipid hydroperoxides in biological fluids

To evaluate the oxidative stress under clinical conditions, a procedure is developed for the chemiluminescence determination of the total concentration of lipid hydroperoxides based on their oxidation in the presence of microperoxidase and chemiluminescence activator isoluminol in a borate buffer so...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2017-07, Vol.72 (7), p.751-755
Main Authors: Proskurnina, E. V., Dzhatdoeva, A. A., Lobichenko, E. N., Shalina, R. I., Vladimirov, Yu. A.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Body fluids
Buffers
Chemical properties
Chemiluminescence
Chemistry
Chemistry and Materials Science
Fertilization
Lipids
Lipoproteins
Measurement
Oxidation
Patients
Peroxides
Stress concentration
Vitamin C
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Summary:To evaluate the oxidative stress under clinical conditions, a procedure is developed for the chemiluminescence determination of the total concentration of lipid hydroperoxides based on their oxidation in the presence of microperoxidase and chemiluminescence activator isoluminol in a borate buffer solution (pH 10.0). The limit of detection for linoleic acid hydroperoxide is 16 nM. The procedure is used to determine lipid hydroperoxides in follicular fluid lipoproteins and in plasma of patients undergoing extracorporeal fertilization.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934817050094