Plant growth promotion and suppression of charcoal‐rot fungus (Macrophomina phaseolina) in velvet bean (Mucuna pruriens L.) by root nodule bacteria

• Published in: Journal of phytopathology 2017-08, Vol.165 (7-8), p.463-478
• Main Authors: Aeron, Abhinav, Maheshwari, Dinesh Kumar, Dheeman, Shrivardhan, Agarwal, Mohit, Dubey, Ramesh Chand, Bajpai, Vivek K.
• Format: Article
• Language: English
• Subjects: Agar; Antibiotic resistance; Antibiotics; Bacteria; biocontrol; Biomass; Bradyrhizobium diazoefficiens; Calcium; Calcium phosphates; Cell culture; Centrifuging; Charcoal rot; Fertilizers; Filtrate; Fungi; Germination; Iron; Iron deficiency; Legumes; Macrophomina phaseolina; Mucuna pruriens; Multiple cropping; Nodules; Phosphates; Plant growth; Promotion; rhizobia; Root nodules; rRNA 16S; Seed germination; Seeds; Sinorhizobium meliloti; Solubilization; Strains (organisms); Symbionts; Tryptophan; velvet bean
• ISSN: 0931-1785; 1439-0434
• DOI: 10.1111/jph.12581

Root‐nodulating bacteria are intimate associates of legumes. From a pool of rhizobia isolated from root nodules of Mucuna pruriens (Velvet bean/Kaunch), RMP66 and BMP17 were found to be capable of promoting siderophore and IAA production and phosphate solubilization (insoluble tri‐calcium). Both symbionts were studied further to determine their abilities to promote plant growth and to control root‐rot in Mucuna pruriens caused by the pathogenic plant fungus Macrophomina phaseolina. RMP66 and BMP17 were selected based on their excellent inhibitory activities against M. phaseolina (by 78% and 71%, respectively) in dual culture and in agar‐well assays using cell‐free culture filtrate (CFCF) (by 76% and 62%, respectively). Both strains inhibited fungal growth to a greater extent in iron‐deficient medium (51% and 69%) than in iron‐supplemented medium (37% and 0%), respectively. CFCFs of RMP66 and BMP17 obtained from Pikovskaya's broth and tryptophan‐amended YEM broth inhibited fungal growth by 80%‐55% and 70%‐43%, respectively, and were identified as Sinorhizobium meliloti RMP66 and Bradyrhizobium diazoefficiens BMP17 by 16S rDNA sequencing. Centrifuged and pelleted cells harvested from exponentially grown cultures of S. meliloti RMP66 and B. diazoefficiens BMP17 were used to bacterize seeds of M. pruriens, which then showed enhanced seed germination (by up to 17% and 12%, respectively), and subsequent increases in other plant growth parameters in field trials. Considerable increases in seedling vigour indices (62%: 53% and 110%: 130%) and biomass (8%: 13% and 25%: 28%) were also observed for bacterial treatments. Tn5‐mediated antibiotic‐resistant marker strains showed enhanced nodule occupancy by up to 72% and 68%, respectively. This study describes a multifunctional legume nodule rhizobia that could be utilized in multicropping systems under different agroclimatic conditions as a bioinoculant and alternative to fertilizers.