Extractive Spectrophotometric Determination of Ofloxacin and Tinidazole with Bromophenol Blue

• Published in: Asian Journal of Research in Chemistry 2013-07, Vol.6 (7), p.726
• Main Authors: Haribabu, Y, Kutty, Sheeja Velayudhan, Vidhya, P M, Greeshma, S
• Format: Article
• Language: English
• Subjects: Accuracy; Bromophenol blue; Capillary electrophoresis; Drug dosages; Ethanol; Infections; Literature reviews; Methods; Pharmaceuticals; Spectrophotometry
• ISSN: 0974-4169; 0974-4150

The study aims to develop a simple, sensitive,rapid,accurate and precise extractive spectrophotometric method for the determination of ofloxacin and tinidazole in bulk drug and tablet dosage form. Recoveries obtained do not differ significantly from 100% showed that there was no interference from the common excipients used in the tablet formulation indicating accuracy and reliability of the method.The proposed method was successfully applied for the determination of ofloxacin and tinidazole in commercial tablet containing 200 mg of ofloxacin and 300 mg of tinidazole ( oflox TZ ). Some methods have also been reported for the determination of TNZ in combination with other drugs including spectrophotometric, capillary electrophoresis and differential pulse polarography [7].Literature survey revealed that spectrophotometric and HPLC methods have been reported for the estimation of OFL and TNZ in pharmaceutical formulations [8-11]. Preparation of phthalate buffer 1.020g of potassium hydrogen phthalate was dissolved in little amount of water and the ph of the solution was adjusted to ph3.6by the addition of 0.1M Hcl. Recoveries obtained do not differ significantly from 100% showed that there was no interference from the common excipients used in the tablet formulation indicating accuracy and reliability of the method [Table 7]. The sample recoveries in all formulation were in good agreement with their respective label claims without the interference of excipients and the other additives. [...]the proposed method can be used for the routine analysis of the samples in pure and its dosage forms. The authors are thankful to the Torrent pharmaceuticals Ltd, Ahmadabad for providing...