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Increased intraocular pressure causes deficiency in the level of ELAVL1/HuR cytoplasmic fraction in the retina

Purpose To evaluate impact of raised intraocular pressure on RNA binding protein ELAVL1/HuR expression in retina. Methods In order to induce glaucomatous damage, intraocular pressure was increased unilaterally using modified rat bead model and maintained for up to 8 weeks. Fellow eye was used as a h...

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Bibliographic Details
Published in:Acta ophthalmologica (Oxford, England) England), 2016-10, Vol.94 (S256), p.n/a
Main Authors: Smedowski, A., Trzeciecka, A., Podracka, L., Pietrucha‐Dutczak, M., Lewin‐Kowalik, J., Akhtar, S., Kaarniranta, K., Amadio, M.
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Language:English
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Summary:Purpose To evaluate impact of raised intraocular pressure on RNA binding protein ELAVL1/HuR expression in retina. Methods In order to induce glaucomatous damage, intraocular pressure was increased unilaterally using modified rat bead model and maintained for up to 8 weeks. Fellow eye was used as a healthy control. Animals were sacrificed, retina and optic nerves were collected and processed for western blot (WB) analysis or for immunostainings. Results Glaucomatous damage was confirmed in electron microscopy by the presence of axonal damage, myelin sheat dezintegration and glial cells proliferation within the optic nerve. Eight weeks glaucoma induced up to 36% loss of Retinal Ganglion Cells (RGCs) in the retina. In fractionated WB analysis of retinal homogenates, the level of active, cytoplasmic fraction of HuR was decreased approximately 3‐times when compared with healthy tissue (p 
ISSN:1755-375X
1755-3768
DOI:10.1111/j.1755-3768.2016.0545