Tear fluid biomarkers, conjunctival inflammation in glaucoma

Summary Ocular surfaces are protected, nourished and lubricated by tear fluid and are exposed environmental factors, topical ophthalmic drugs. Inflammation and wound healing are vital processes involved in the defense mechanisms and in the pathogenesis of many eye diseases. Chronic topical medicatio...

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Bibliographic Details
Published in:Acta ophthalmologica (Oxford, England) England), 2016-10, Vol.94 (S256), p.n/a
Main Author: Uusitalo, H.
Format: Article
Language:English
Subjects:
Biomarkers
Conjunctiva
Defense mechanisms
Diagnostic software
Environmental factors
Eye
Eye diseases
Glaucoma
Inflammation
Ophthalmology
Proteins
Proteomics
Surface chemistry
Surgery
Wound healing
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Summary:Summary Ocular surfaces are protected, nourished and lubricated by tear fluid and are exposed environmental factors, topical ophthalmic drugs. Inflammation and wound healing are vital processes involved in the defense mechanisms and in the pathogenesis of many eye diseases. Chronic topical medication is known to affect ocular surface eliciting inflammation, activating wound healing and worsening the success of glaucoma surgery. We used proteomics as a powerful tool to diagnose ocular surface diseases and detect pathogenic mechanisms and biomarkers in glaucoma patients. Tear fluid and conjunctival tissue specimens of glaucoma and control patients were analyzed by using proteomic and histological techniques. Proteomic analyses were performed with NanoLC‐TripleMSTOF MS using SWATH acquisition. For library generation UniProtKB/SwissProt database was used. Proteomic analysis of conjunctiva demonstrates protein profile of >2,000 proteins. Pathway analysis allowed analysis of processes like inflammation and wound healing. It will also give an opportunity to further analyze the role of pathogenic mechanisms leading to failure in glaucoma surgery and to develop novel diagnostic tools, biomarkers and therapies for glaucoma patients.
ISSN:1755-375X
1755-3768
DOI:10.1111/j.1755-3768.2016.0194