Nickel oxide nanoparticles induced pulmonary fibrosis via TGF-[beta]1 activation in rats

Nano nickel oxide (NiO), widely used in industry, has recently been discovered to have pulmonary toxicity. However, no subchronic exposure studies about nano NiO-induced pulmonary fibrosis have been reported. The objective of this study was to investigate pulmonary fibrosis induced by nano NiO and i...

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Bibliographic Details
Published in:Human & experimental toxicology 2017-08, Vol.36 (8), p.802
Main Authors: Chang, XH, Zhu, A, Liu, FF, Zou, LY, Su, L, Liu, SK, Zhou, HH, Sun, YY, Han, AJ, Sun, YF, Li, S, Li, J, Sun, YB
Format: Article
Language:English
Subjects:
Activation
Collagen
Enzyme-linked immunosorbent assay
Exposure
Fibrosis
Gene expression
Hydroxyproline
Lung diseases
Matrix metalloproteinase
Metalloproteinase
Nanoparticles
Nanostructure
Nickel
Polymerase chain reaction
Pulmonary fibrosis
Rats
Reverse transcription
Ribonucleic acid
RNA
Rodents
Smad2 protein
Smad4 protein
Staining
Toxicity
Trachea
Transforming growth factor-b1
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Summary:Nano nickel oxide (NiO), widely used in industry, has recently been discovered to have pulmonary toxicity. However, no subchronic exposure studies about nano NiO-induced pulmonary fibrosis have been reported. The objective of this study was to investigate pulmonary fibrosis induced by nano NiO and its potential mechanism in rats. Male Wistar rats (n = 40, 200-240 g) were randomized into control group, nano NiO groups (0.015, 0.06, and 0.24 mg/kg), and micro NiO group (0.024 mg/kg). All rats were killed to collect lung tissue after intratracheal instillation of NiO particles twice a week for 6 weeks. To identify pulmonary fibrosis, Masson trichrome staining, hydroxyproline content, and collagen protein expression were performed. The results showed widespread lung fibrotic injury in histological examination and increased content of hydroxyproline, collagen types I and III in rat lung tissue exposed to nano NiO. To explore the potential pulmonary fibrosis mechanism, transforming growth factor beta 1 (TGF-β 1) content was measured by enzyme-linked immunosorbent assay, and the messenger RNA expression of key indicators was detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The TGF-β 1 content was increased in nano NiO exposure groups, as well as the upregulated gene expression of TGF-β 1, Smad2, Smad4, matrix metalloproteinase, and tissue inhibitor of metalloproteinase. The findings indicated that nano NiO could induce pulmonary fibrosis, which may be related to TGF-β 1 activation.
ISSN:0960-3271
1477-0903
DOI:10.1177/0960327116666650