Determination of genotoxic effects of methidathion alkaline hydrolysis in human lymphocytes using the micronucleus assay and square-wave voltammetry

The interaction of pesticides with environmental factors, such as pH, may result in alterations of their physicochemical properties and should be taken into consideration in regard to their classification. This study investigates the genotoxicity of methidathion and its alkaline hydrolysis by-produc...

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Bibliographic Details
Published in:Bioelectrochemistry (Amsterdam, Netherlands) Netherlands), 2017-02, Vol.113, p.9-14
Main Authors: Stivaktakis, Polychronis D., Giannakopoulos, Evangelos, Vlastos, Dimitris, Matthopoulos, Demetrios P.
Format: Article
Language:English
Subjects:
Adsorptivity
Adult
Agrochemicals
Assaying
Byproducts
Cell Membrane - drug effects
Cytokinesis
Cytokinesis - drug effects
Electrochemistry
Electrodynamics
Environmental factors
Genotoxicity
HMDE
Humans
Hydrologic data
Hydrolysis
Lymphocytes
Lymphocytes - cytology
Lymphocytes - drug effects
Methidathion
Micronuclei
Micronucleus assay
Micronucleus Tests
Mutagenicity Tests
Organothiophosphorus Compounds - chemistry
Organothiophosphorus Compounds - metabolism
Organothiophosphorus Compounds - toxicity
Pesticides
pH effects
Physicochemical properties
Square-wave voltammetry
Stripping
Voltammetry
Young Adult
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Summary:The interaction of pesticides with environmental factors, such as pH, may result in alterations of their physicochemical properties and should be taken into consideration in regard to their classification. This study investigates the genotoxicity of methidathion and its alkaline hydrolysis by-products in cultured human lymphocytes, using the square-wave voltammetry (square wave-adsorptive cathodic stripping voltammetry (SW-AdCSV) technique) and the cytokinesis block micronucleus assay (CBMN assay). According to the SW-AdCSV data the alkaline hydrolysis of methidathion results in two new molecules, one non-electro-active and a second electro-active which is more genotoxic than methidathion itself in cultured human lymphocytes, inducing higher micronuclei frequencies. The present study confirms the SW-AdCSV technique as a voltammetric method which can successfully simulates the electrodynamics of the cellular membrane. [Display omitted] •Genotoxicity of methidathion and its alkaline hydrolysis by-products were evaluated.•The SW-AdCSV technique and the CBMN assay in human lymphocytes were used.•Alkaline hydrolysis results in a non-electro-active and an electro-active molecule.•The electro-active molecule is more genotoxic than methidathion in human cells.•HMDE electrodynamically simulates the cellular membrane.
ISSN:1567-5394
1878-562X
DOI:10.1016/j.bioelechem.2016.08.004