Resveratrol enhances brown adipocyte formation and function by activating AMP-activated protein kinase (AMPK) [alpha]1 in mice fed high-fat diet

Scope Enhancing the formation and function of brown adipose tissue (BAT) increases thermogenesis and hence reduces obesity. Thus, we investigate the effects of resveratrol (Resv) on brown adipocyte formation and function in mouse interscapular BAT (iBAT). Methods and results CD1 mice and stromal vas...

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Bibliographic Details
Published in:Molecular nutrition & food research 2017-04, Vol.61 (4)
Main Authors: Wang, Songbo, Liang, Xingwei, Yang, Qiyuan, Fu, Xing, Zhu, Meijun, Rodgers, B D, Jiang, Qingyan, Dodson, Michael V, Du, Min
Format: Article
Language:English
Subjects:
Adipocytes
Adipose tissue
Adipose tissue (brown)
AMP
AMP-activated protein kinase
Cytochrome
Cytochrome c
Gene expression
High fat diet
In vitro methods and tests
Kinases
Markers
Mice
Obesity
Oxygen consumption
Proteins
Pyruvic acid
Resveratrol
Thermogenesis
Uncoupling protein 1
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Summary:Scope Enhancing the formation and function of brown adipose tissue (BAT) increases thermogenesis and hence reduces obesity. Thus, we investigate the effects of resveratrol (Resv) on brown adipocyte formation and function in mouse interscapular BAT (iBAT). Methods and results CD1 mice and stromal vascular cells (SVCs) isolated from iBAT were treated with Resv. Expression of brown adipogenic and thermogenic markers, and involvement of AMP-activated protein kinase (AMPK)[alpha]1 were assessed. In vivo, Resv-enhanced expression of brown adipogenic markers, PR domain-containing 16 (PRDM16) and thermogenic genes, uncoupling protein 1 (UCP1) and cytochrome C in iBAT, along with smaller lipid droplets, elevated AMPK[alpha] activity and increased oxygen consumption. Meanwhile, Resv promoted expression of PRDM16, UCP1, PGC1[alpha], cytochrome C and pyruvate dehydrogenase (PDH) in differentiated iBAT SVCs, suggesting that Resv enhanced brown adipocyte formation and function in vitro. In addition, Resv stimulated AMPK[alpha] and oxygen consumption in differentiated iBAT SVCs. However, the promotional effects of Resv were diminished by AMPK inhibition or AMPK[alpha]1 knockout, implying the involvement of AMPK[alpha]1 in this process. Conclusion Resv enhanced brown adipocyte formation and thermogenic function in mouse iBAT by promoting the expression of brown adipogenic markers via activating AMPK[alpha]1, which contributed to the anti-obesity effects of Resv.
ISSN:1613-4125
1613-4133
DOI:10.1002/mnfr.201600746