Spontaneous mouse mammary tumor cell lysates induce IgG production in spleen mononuclear cells of healthy and tumor-bearing mice

Background. We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo. Methods. The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence...

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Published in:Journal of immunoassay & immunochemistry 2017-05, Vol.38 (3), p.333-342
Main Authors: Khalilnezhad, Ahad, Mahmoudian, Elham, Mosaffa, Nariman, Mohsenifar, Jaleh, Amani, Davar
Format: Article
Language:English
Subjects:
Animal models
Animals
Cell culture
Enzyme-Linked Immunosorbent Assay
Female
Humoral immunity
Immunity
immunoglobulin
Immunoglobulin G
Immunoglobulin G - biosynthesis
Immunoglobulin G - blood
Immunoglobulin G - immunology
Interferon-gamma - blood
Interleukin 4
Interleukin-4 - blood
Leukocytes (mononuclear)
Lysates
Mammary gland
Mammary Neoplasms, Animal - immunology
Mammary Neoplasms, Animal - pathology
Mice
Mice, Inbred BALB C
Rodents
Spleen
Spleen - cytology
Spleen - immunology
tumor cell lysate
Tumor Cells, Cultured
Tumors
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container_issue 3
container_start_page 333
container_title Journal of immunoassay & immunochemistry
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creator Khalilnezhad, Ahad
Mahmoudian, Elham
Mosaffa, Nariman
Mohsenifar, Jaleh
Amani, Davar
description Background. We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo. Methods. The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence of TCL and mitogen for 42 hr at 37°C, separately. Serum and SMCs culture supernatant levels of IFNγ, IL-4, and total IgG were measured using special ELISA kits. Results. Serum IgG level of TBM was significantly higher than that of HM group (P = 0.019), while serum IFNγ and IL-4 did not differ between two groups (P > 0.05). Mitogen significantly induced ex vivo production of both IFNγ (P = 0.013) and IL-4 (P = 0.015) by SMCs from HM group, and only IL-4 (P = 0.049) by SMCs from TBM group. In contrast, TCL increased ex vivo production of IgG by SMCs from both HM (P = 0.034) and TBM (P = 0.016) groups. The ex vivo IgG revealed a moderate positive correlation with tumor size (r = 0.578, P = 0.422). Conclusion. It seems that TCL prepared from SMMT are potent inducers of IgG production. This may propose TCL as a potential tool for monitoring of humoral immunity in animal models.
doi_str_mv 10.1080/15321819.2016.1266499
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We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo. Methods. The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence of TCL and mitogen for 42 hr at 37°C, separately. Serum and SMCs culture supernatant levels of IFNγ, IL-4, and total IgG were measured using special ELISA kits. Results. Serum IgG level of TBM was significantly higher than that of HM group (P = 0.019), while serum IFNγ and IL-4 did not differ between two groups (P &gt; 0.05). Mitogen significantly induced ex vivo production of both IFNγ (P = 0.013) and IL-4 (P = 0.015) by SMCs from HM group, and only IL-4 (P = 0.049) by SMCs from TBM group. In contrast, TCL increased ex vivo production of IgG by SMCs from both HM (P = 0.034) and TBM (P = 0.016) groups. The ex vivo IgG revealed a moderate positive correlation with tumor size (r = 0.578, P = 0.422). Conclusion. It seems that TCL prepared from SMMT are potent inducers of IgG production. This may propose TCL as a potential tool for monitoring of humoral immunity in animal models.</description><identifier>ISSN: 1532-1819</identifier><identifier>EISSN: 1532-4230</identifier><identifier>DOI: 10.1080/15321819.2016.1266499</identifier><identifier>PMID: 27929756</identifier><language>eng</language><publisher>England: Taylor &amp; Francis</publisher><subject>Animal models ; Animals ; Cell culture ; Enzyme-Linked Immunosorbent Assay ; Female ; Humoral immunity ; Immunity ; immunoglobulin ; Immunoglobulin G ; Immunoglobulin G - biosynthesis ; Immunoglobulin G - blood ; Immunoglobulin G - immunology ; Interferon-gamma - blood ; Interleukin 4 ; Interleukin-4 - blood ; Leukocytes (mononuclear) ; Lysates ; Mammary gland ; Mammary Neoplasms, Animal - immunology ; Mammary Neoplasms, Animal - pathology ; Mice ; Mice, Inbred BALB C ; Rodents ; Spleen ; Spleen - cytology ; Spleen - immunology ; tumor cell lysate ; Tumor Cells, Cultured ; Tumors</subject><ispartof>Journal of immunoassay &amp; immunochemistry, 2017-05, Vol.38 (3), p.333-342</ispartof><rights>2017 Taylor &amp; Francis 2017</rights><rights>2017 Taylor &amp; Francis</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c309t-3c638533fd0aa1c1f6471a0cee50bbbd7c04ec65b2df0a4d5234c9006a2606703</citedby><cites>FETCH-LOGICAL-c309t-3c638533fd0aa1c1f6471a0cee50bbbd7c04ec65b2df0a4d5234c9006a2606703</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27929756$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Khalilnezhad, Ahad</creatorcontrib><creatorcontrib>Mahmoudian, Elham</creatorcontrib><creatorcontrib>Mosaffa, Nariman</creatorcontrib><creatorcontrib>Mohsenifar, Jaleh</creatorcontrib><creatorcontrib>Amani, Davar</creatorcontrib><title>Spontaneous mouse mammary tumor cell lysates induce IgG production in spleen mononuclear cells of healthy and tumor-bearing mice</title><title>Journal of immunoassay &amp; immunochemistry</title><addtitle>J Immunoassay Immunochem</addtitle><description>Background. We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo. Methods. The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence of TCL and mitogen for 42 hr at 37°C, separately. Serum and SMCs culture supernatant levels of IFNγ, IL-4, and total IgG were measured using special ELISA kits. Results. Serum IgG level of TBM was significantly higher than that of HM group (P = 0.019), while serum IFNγ and IL-4 did not differ between two groups (P &gt; 0.05). Mitogen significantly induced ex vivo production of both IFNγ (P = 0.013) and IL-4 (P = 0.015) by SMCs from HM group, and only IL-4 (P = 0.049) by SMCs from TBM group. In contrast, TCL increased ex vivo production of IgG by SMCs from both HM (P = 0.034) and TBM (P = 0.016) groups. The ex vivo IgG revealed a moderate positive correlation with tumor size (r = 0.578, P = 0.422). 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immunochemistry</jtitle><addtitle>J Immunoassay Immunochem</addtitle><date>2017-05-04</date><risdate>2017</risdate><volume>38</volume><issue>3</issue><spage>333</spage><epage>342</epage><pages>333-342</pages><issn>1532-1819</issn><eissn>1532-4230</eissn><abstract>Background. We hypothesized that Tumor cell lysate (TCL) prepared from spontaneous mouse mammary tumor (SMMT) may elicit IgG production by spleen mononuclear cells (SMCs) in ex vivo. Methods. The SMCs from healthy mice (HM, n = 6) and four-week SMMT-bearing mice (TBM, n = 6) was cultured in presence of TCL and mitogen for 42 hr at 37°C, separately. Serum and SMCs culture supernatant levels of IFNγ, IL-4, and total IgG were measured using special ELISA kits. Results. Serum IgG level of TBM was significantly higher than that of HM group (P = 0.019), while serum IFNγ and IL-4 did not differ between two groups (P &gt; 0.05). Mitogen significantly induced ex vivo production of both IFNγ (P = 0.013) and IL-4 (P = 0.015) by SMCs from HM group, and only IL-4 (P = 0.049) by SMCs from TBM group. In contrast, TCL increased ex vivo production of IgG by SMCs from both HM (P = 0.034) and TBM (P = 0.016) groups. The ex vivo IgG revealed a moderate positive correlation with tumor size (r = 0.578, P = 0.422). Conclusion. It seems that TCL prepared from SMMT are potent inducers of IgG production. This may propose TCL as a potential tool for monitoring of humoral immunity in animal models.</abstract><cop>England</cop><pub>Taylor &amp; Francis</pub><pmid>27929756</pmid><doi>10.1080/15321819.2016.1266499</doi><tpages>10</tpages></addata></record>
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1532-4230
language eng
recordid cdi_proquest_journals_1923346393
source Taylor and Francis Science and Technology Collection
subjects Animal models
Animals
Cell culture
Enzyme-Linked Immunosorbent Assay
Female
Humoral immunity
Immunity
immunoglobulin
Immunoglobulin G
Immunoglobulin G - biosynthesis
Immunoglobulin G - blood
Immunoglobulin G - immunology
Interferon-gamma - blood
Interleukin 4
Interleukin-4 - blood
Leukocytes (mononuclear)
Lysates
Mammary gland
Mammary Neoplasms, Animal - immunology
Mammary Neoplasms, Animal - pathology
Mice
Mice, Inbred BALB C
Rodents
Spleen
Spleen - cytology
Spleen - immunology
tumor cell lysate
Tumor Cells, Cultured
Tumors
title Spontaneous mouse mammary tumor cell lysates induce IgG production in spleen mononuclear cells of healthy and tumor-bearing mice
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