Mg2+‐Dependent High Mechanical Anisotropy of Three‐Way‐Junction pRNA as Revealed by Single‐Molecule Force Spectroscopy

Mechanical anisotropy is ubiquitous in biological tissues but is hard to reproduce in synthetic biomaterials. Developing molecular building blocks with anisotropic mechanical response is the key towards engineering anisotropic biomaterials. The three‐way‐junction (3WJ) pRNA, derived from ϕ29 DNA pac...

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Bibliographic Details
Published in:Angewandte Chemie 2017-08, Vol.129 (32), p.9504-9508
Main Authors: Sun, Yang, Di, Weishuai, Li, Yiran, Huang, Wenmao, Wang, Xin, Qin, Meng, Wang, Wei, Cao, Yi
Format: Article
Language:eng ; jpn
Subjects:
Alloys
Anisotropy
Binding sites
Biomaterialiendesign
Biomaterials
Biomedical materials
Chemistry
Construction
Deoxyribonucleic acid
DNA
Engineering
Magnesium
Mechanical analysis
Mechanische Anisotropie
Motor task performance
Packaging
pRNA
Rasterkraftmikroskopie
Rupture
Rupturing
Spectroscopy
Surgical implants
Tissues
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Summary:Mechanical anisotropy is ubiquitous in biological tissues but is hard to reproduce in synthetic biomaterials. Developing molecular building blocks with anisotropic mechanical response is the key towards engineering anisotropic biomaterials. The three‐way‐junction (3WJ) pRNA, derived from ϕ29 DNA packaging motor, shows strong mechanical anisotropy upon Mg2+ binding. In the absence of Mg2+, 3WJ‐pRNA is mechanically weak without noticeable mechanical anisotropy. In the presence of Mg2+, the unfolding forces can differ by more than 4‐fold along different pulling directions, ranging from about 47 pN to about 219 pN. Mechanical anisotropy of 3WJ‐pRNA stems from pulling direction dependent cooperativity for the rupture of two Mg2+ binding sites, which is a novel mechanism for the mechanical anisotropy of biomacromolecules. It is anticipated that 3WJ‐pRNA can be used as a key element for the construction of biomaterials with controllable mechanical anisotropy. Eine RNA‐Dreiwegekreuzung (3WJ‐pRNA) weist abhängig von der Kraftrichtung Entfaltungskräfte zwischen 47 und 219 pN auf. Diese mechanische Anisotropie hat ihren Ursprung in der unterschiedlichen Kooperativität für das Aufbrechen zweier Mg2+‐Bindestellen. Es ist vorstellbar, dass 3WJ‐pRNA für den Aufbau von Biomaterialien mit einstellbarer mechanischer Anisotropie genutzt werden kann.
ISSN:0044-8249
1521-3757
DOI:10.1002/ange.201704113