Localization of nucleoside transporters in rat epididymis

The epididymis relies on transporters for the secretion of nucleosides and influence the disposition of nucleoside analogs (NSA). Since these compounds can cross the blood–testis barrier (BTB), it is important to understand if the epididymis reabsorbs NSA drugs. The purpose of this study is to deter...

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Bibliographic Details
Published in:Journal of biochemical and molecular toxicology 2017-08, Vol.31 (8), p.n/a
Main Authors: Klein, David M., Harding, Marcus C., Crowther, Meghan K, Cherrington, Nathan J.
Format: Article
Language:English
Subjects:
Animals
Balancing
Basal cells
Blood
Blood-Testis Barrier - enzymology
Carrier Proteins - metabolism
Drugs
Epididymis
Epididymis - cytology
Epididymis - enzymology
Epithelial cells
Epithelium
Equilibrative Nucleoside Transporter 1
Immunohistochemistry
Localization
Male
male genital tract
Membrane Transport Proteins - metabolism
Nuclei
Nuclei (cytology)
Nucleoside analogs
nucleoside transport
Nucleoside transporter
Nucleosides
Nucleosides - pharmacokinetics
Nucleosides - pharmacology
Rats
Rats, Sprague-Dawley
Reabsorption
reproductive toxicology
Rodents
xenobiotic transporter
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Summary:The epididymis relies on transporters for the secretion of nucleosides and influence the disposition of nucleoside analogs (NSA). Since these compounds can cross the blood–testis barrier (BTB), it is important to understand if the epididymis reabsorbs NSA drugs. The purpose of this study is to determine the localization of nucleoside transporters expressed within rat epididymis to demonstrate the potential of epididymal reabsorption. Using immunohistochemistry, we determined that equilibrative nucleoside transporter 1 (ENT1) is localized to the basolateral membrane of epithelial cells, ENT2 is expressed in the nucleus of the epithelium and CNT2 is expressed by basal cells. The expression pattern for these transporters suggests that nucleosides are able to access the epithelial cells of the epididymal duct via the blood, but not from the lumen. We did not find any evidence for a transepithelial reabsorption pathway indicating the NSA drugs that cross the BTB remain within the epididymis.
ISSN:1095-6670
1099-0461
DOI:10.1002/jbt.21911