Karyotypic study of five Lutjanid species using conventional and Ag-NORs banding techniques

• Published in: Cytology and genetics 2017-07, Vol.51 (4), p.315-324
• Main Authors: Phimphan, Sumalee, Supiwong, Weerayuth, Tanomtong, Alongklod, Pinthong, Krit, Sangpakdee, Wiwat, Kaewsri, Sarawut
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Chromosomes; Human Genetics; Nucleoli; Species
• ISSN: 0095-4527; 1934-9440
• DOI: 10.3103/S0095452717040077

The first cytogenetic comparisons of five snapper species from Thailand were presented here. Renal cell samples were taken from blacktail snapper ( Lutjanus fulvus ), five lined snapper ( L. quinquelineatus ), dory snapper ( L. fulviflamma ), brownstripe red snapper ( L. vitta ), and mangrove red snapper ( L. argentimaculatus ). The mitotic chromosome preparation was prepared directly from kidney cells. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results exhibited that all five snapper species have the diploid chromosome numbers of 2 n = 48 and the fundamental numbers (NF) of 48. The presences of large, medium, and small telocentric chromosomes were 22-24-2, 24-20-4, 36-10-2, 28-16-4 and 36-10-2, respectively. The Ag- NORs banding technique provides the pair of nucleolar organizer regions (NORs) at subcentromeric region of the long arm of the respective telocentric chromosome pairs 9, 1, 3, 4 and 9. Their karyotype formulas is as follows: L. fulvus (2 n = 48): L 22 t + M 24 t + S 2 t , L. quinquelineatus (2 n = 48): L 24 t + M 20 t + S 4 t , L. fulviflamma (2 n = 48): Lt36 + Mt10 + St2, L. vitta (2 n = 48): L 28 t + M 16 t + S 4 t , and L. argentimaculatus (2 n = 48): L 36 t + M 10 t + S 2 t .