Electrochemiluminescence immunoassay for the carcinoembryonic antigen using CdSe:Eu nanocrystals

• Published in: Mikrochimica acta (1966) 2017-05, Vol.184 (5), p.1353-1360
• Main Authors: Liu, Qiao, Liu, Xing-Pei, Wei, Yu-Pin, Mao, Chang-Jie, Niu, He-Lin, Song, Ji-Ming, Jin, Bao-Kang, Zhang, Sheng-Yi
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Antigens; CEA (Oncology); Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Colon cancer; Electrochemiluminescence; Electron microscopy; Electrons; Immunoassay; Immunosensors; Microengineering; Nanochemistry; Nanocrystals; Nanostructure; Nanotechnology; Original Paper; Pancreatic cancer; Photoelectricity; Transmission electron microscopy; X ray powder diffraction; X-ray diffraction
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-017-2114-6

CdSe:Eu nanocrystals were successfully synthesized and characterized by transmission electron microscopy, X-ray powder diffraction, and X-ray photoelectric spectroscopy. The CdSe:Eu nanocrystals showed enhanced green electrochemiluminescence (ECL) intensity when compared to pure CdSe nanocrystals. Further, the nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen (CEA) that has a linear response over the 1.0 fg·mL −1 to 100 ng·mL −1  CEA concentration range with a 0.4 fg·mL −1 detection limit. The assay was applied to the determination of CEA in human serum samples. Graphical abstract Schematic of the assay: GCE-glassy-carbon electrode, Ab- Antibody, BSA- Bovine serum albumin, Ag- Antigen. CdSe:Eu nanocrystals were used to design an ECL immunosensor for the detection of carcinoembryonic antigen.