The application of yeast hybrid systems in protein interaction analysis

Yeast hybrid systems have been widely used due to their convenience and low cost. Based on these systems, many methods have been developed to analyze protein–protein, protein–DNA and protein–RNA interactions. In this paper, we are reviewing these different yeast hybrid systems. According to the numb...

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Bibliographic Details
Published in:Molecular biology (New York) 2016-09, Vol.50 (5), p.663-670
Main Authors: Zhu, Z.-X., Yu, Z.-M., Taylor, J. L., Wu, Y.-H., Ni, J.
Format: Article
Language:English
Subjects:
Binding sites
Biochemistry
Biomedical and Life Sciences
Cytosol
Deoxyribonucleic acid
DNA
Human Genetics
Life Sciences
Localization
Membrane proteins
Protein interaction
Proteins
Reviews
Ribonucleic acid
RNA
Technological planning
Yeast
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Summary:Yeast hybrid systems have been widely used due to their convenience and low cost. Based on these systems, many methods have been developed to analyze protein–protein, protein–DNA and protein–RNA interactions. In this paper, we are reviewing these different yeast hybrid systems. According to the number of hybrid proteins, yeast hybrid systems can be divided into three categories, yeast one-hybrid, yeast two-hybrid and yeast three-hybrid systems. Alternatively, yeast hybrid systems can be categorized according to the subcellular localization of the protein interaction process in the cell into nuclear protein–protein interactions, cytosol protein–protein interactions and membrane protein–protein interactions. Throughout the review, we focus on the progress and limitations of each yeast hybrid system over the recent years.
ISSN:0026-8933
1608-3245
DOI:10.1134/S0026893316050186