C78 FIBROSIS: MEDIATORS AND MODULATORS: Bleomycin Induced Epithelial Mesenchymal Transition In Mice

In vitro, it has been already shown that BLM induced EMT via the TGF-ß/Smad signaling pathway in human alveolar epithelial cells.· However, implication of EMT in BLM-induced lung fibrosis in mice has not been fully clarified. Aim Our aim is to examine epithelial and mesenchymal markers, phospho-Smad...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2017-01, Vol.195
Main Authors: Kato, M, Takahashi, F, Ihara, H, Tajima, K, Sato, T, Bascoro, H, Wirawan, A, Hidayat, M, Takeda, I, Hayakawa, D, Kanemaru, R, Koinuma, Y, Suina, K, Shimada, N, Sasaki, S, Takahashi, K
Format: Article
Language:English
Subjects:
Lungs
Pulmonary fibrosis
Rodents
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Summary:In vitro, it has been already shown that BLM induced EMT via the TGF-ß/Smad signaling pathway in human alveolar epithelial cells.· However, implication of EMT in BLM-induced lung fibrosis in mice has not been fully clarified. Aim Our aim is to examine epithelial and mesenchymal markers, phospho-Smad2, and TGFß-induced transcriptional factor in the lungs of BLM-treated mice by immunohistochemical analysis and quantitative real time polymerase chain reaction (qPCR).
ISSN:1073-449X
1535-4970