C15 WHEN MICROBES ATTACK: HOST-PATHOGEN INTERACTIONS: Influenza A Virus Relocalizes Na,k-Atpase In Vitro And In Vivo To Support Its Replication - Impact On Lung Injury And Alveolar Edema Clearance

Methods To study the effects of IAV-infection on the Na,K-ATPase, Calu-3 as well as primary isolated alveolar epithelial cells (AEC) were infected in vitro with A/PR/8/1934 (PR8) or transfected with single viral proteins. Conclusion We provide evidence that in infected cells, in contrast to non-infe...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2017-01, Vol.195
Main Authors: Peteranderl, C, Lecuona, E, Kuznetsova, I, Wolff, T, Morty, R, Lohmeyer, J, Seeger, W, Pleschka, S, Mutlu, G M, Budinger, G S, Sznajder, J I, Herold, S V
Format: Article
Language:English
Subjects:
Antiviral drugs
Edema
Infections
Influenza
Localization
Rodents
Sodium
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Summary:Methods To study the effects of IAV-infection on the Na,K-ATPase, Calu-3 as well as primary isolated alveolar epithelial cells (AEC) were infected in vitro with A/PR/8/1934 (PR8) or transfected with single viral proteins. Conclusion We provide evidence that in infected cells, in contrast to non-infected bystander cells, Na,K-ATPase expression is not reduced by an interferon-driven, probably antiviral signaling event, but is relocalized to the apical cell membrane upon an interaction with the viral M2 protein.
ISSN:1073-449X
1535-4970