A77 CHANGES IN THE LUNG DUE TO DIETARY AND ENVIRONMENTAL EXPOSURES: Targeting Nicotinic Receptors For Asthma

ASM samples are from lung specimens obtained incidental to patient thoracic surgery at Mayo Clinic Rochester (medical histories used to identify patient demographics, asthma status etc.). Using western blot analysis, we demonstrated that the a7 subunit is substantially expressed in ASM tissue and fu...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2017-01, Vol.195
Main Authors: Pabelick, C, Thompson, M, Sine, S, Prakash, Y S
Format: Article
Language:English
Subjects:
Asthma
Nicotine
Thoracic surgery
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Summary:ASM samples are from lung specimens obtained incidental to patient thoracic surgery at Mayo Clinic Rochester (medical histories used to identify patient demographics, asthma status etc.). Using western blot analysis, we demonstrated that the a7 subunit is substantially expressed in ASM tissue and further increased in ASM from moderate asthmatics, and exposure to nicotine, CS extract (CSE) or dissolved e-cig vapor. 10 pM nicotine enhances agonist-induced [Ca2+]cyť and involves Ca2+ influx (suggested by siRNAs against store operated Ca2+mechanisms such as TRPC3 and Orai1.
ISSN:1073-449X
1535-4970