D108 CELL FATE DECISIONS: SENESCENCE, REPAIR, AND REGENERATION: Distal Pneumocyte Differentiation Of Basal Epithelial Stem Cells By Inhibition Of Notch Signaling During Ex Vivo Lung Regeneration

BESCs cultured on human acellular human lung slices in vitro also up-regulated SP-C expression following 5 days of Notch inhibition treatment (3.03±0.17-fold increase vs. untreated). Additionally, BESCs grown in 3D spheroid culture also upregulated SP-C following Notch inhibition (5.32±0.89-fold inc...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2017-01, Vol.195
Main Authors: Gilpin, S E, Wu, T, Ren, X, Evangelista-Leite, D, Ott, H C
Format: Article
Language:English
Subjects:
Proteins
Rodents
Stem cells
Surfactants
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Summary:BESCs cultured on human acellular human lung slices in vitro also up-regulated SP-C expression following 5 days of Notch inhibition treatment (3.03±0.17-fold increase vs. untreated). Additionally, BESCs grown in 3D spheroid culture also upregulated SP-C following Notch inhibition (5.32±0.89-fold increase) Induction of a distal Type2 pneumocyte fate was then confirmed following delivery of BESCs to the airways of acellular rat lung scaffolds and ex vivo biomimetic culture, with delivery of the inhibitors through the vascular perfusate for 5 days (13.08±1.15-fold increase in SP-C expression).
ISSN:1073-449X
1535-4970