A26 DIAGNOSIS AND TREATMENT OF TUBERCULOSIS: The Use Of Branched-Dna In Situ Hybridization To Diagnose Mycobacterial Infection In Formalin Fixed-Paraffin Embedded Tissues

The thick mycobacterial cell wall has posed a significant barrier to ISH based TB diagnostics. [...]we tested a panel of pre-treatment conditions described in the literature or provided by ACD to find conditions that allow the sensitive and specific detection of mycobacterial nucleic acids. Conclusi...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2017-01, Vol.195
Main Authors: Aguilar Rodriguez, B L, Hussien, R, Hunt, P, McCune, J, Vasquez, J J
Format: Article
Language:English
Subjects:
Deoxyribonucleic acid
DNA
Infections
Medical diagnosis
Tuberculosis
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Summary:The thick mycobacterial cell wall has posed a significant barrier to ISH based TB diagnostics. [...]we tested a panel of pre-treatment conditions described in the literature or provided by ACD to find conditions that allow the sensitive and specific detection of mycobacterial nucleic acids. Conclusion Branched DNA-ISH technology offers an alternative approach for the diagnosis of mycobacteria in FFPE tissues. [...]this technique is easily scalable, rapid, and transferable to cell suspensions and other sample types.
ISSN:1073-449X
1535-4970