A67 BREAKDOWN: THE CELLULAR CONTRIBUTION TO PULMONARY HYPERTENSION: Effect Of Over Expression Of Natriuretic Peptide Receptor-A (npr-A) And Receptor-C (npr-C) On Pulmonary Vascular Endothelial Barrier Function

Intracellular cGMP levels were measured by enzyme inked immunosorbent assay (ELISA): The effect of thrombin, ANP and NPR-A or NPR-C expression on endothelial barrier function was assessed by 3 way ANOVA. Intracellular cGMP levels were similar in all 3 cells under basal conditions (1.24 ± 1.31 versus...

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Bibliographic Details
Published in:American journal of respiratory and critical care medicine 2017-01, Vol.195
Main Authors: Klinger, J R, Duong, H, Duran, L, Liang, O, Kumar, A, Harrington, E O
Format: Article
Language:English
Subjects:
Hypertension
Nitric oxide
Peptides
Rodents
Online Access:Get full text
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Summary:Intracellular cGMP levels were measured by enzyme inked immunosorbent assay (ELISA): The effect of thrombin, ANP and NPR-A or NPR-C expression on endothelial barrier function was assessed by 3 way ANOVA. Intracellular cGMP levels were similar in all 3 cells under basal conditions (1.24 ± 1.31 versus 0.44 ± 0.71 versus 0.51 ± 0.67, pmol/ml for NPR-A, NPR-C, Zs-green, respectively) and increased markedly in response to ANP, with an increase that was approximately 4-fold greater in cells that overexpressed NPR-A as compared to those that overexpressed Zs-green or NPR-C (523.4 ± 466.9 versus 166.2 ± 124.2 versus 136.6 ± 118.8 pmol/ml).
ISSN:1073-449X
1535-4970