Characterization of a long isoform of IL-1R8 (TIR8/SIGIRR)

IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence betwe...

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Bibliographic Details
Published in:European cytokine network (Montrouge) 2017-06, Vol.28 (2), p.63-69
Main Authors: Vilia, Maria Giovanna, Tocchetti, Marta, Fonte, Eleonora, Sana, Ilenia, Muzio, Marta
Format: Article
Language:English
Subjects:
Alternative splicing
Cell lines
Exons
Inflammation
Interleukin 1
Interleukin 1 receptors
Isoforms
Localization
Membrane proteins
mRNA
Proteins
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Summary:IL-1R8, also known as SIGIRR or TIR8, is a trans-membrane protein belonging to the IL-1 receptor family. The human gene includes ten exons, and alternative splicing can result in different isoforms. We, herein, characterized a longer isoform of IL-1R8 containing an in-frame additional sequence between the TIR domain and the C-terminal portion of the protein. IL-1R8 Long (IL-1R8L1) mRNA was specifically expressed and regulated in distinct cell lines, in a manner similar to the classic isoform. Overexpression of IL-1R8L1 resulted in the production of a corresponding protein that showed a pattern of cell localization similar to the classic isoform. An antibody directed against an IL-1R8L1 specific peptide, detected this novel isoform in different cell lines and tissues where this protein may complement the anti-inflammatory functions of classic IL-1R8.
ISSN:1148-5493
1952-4005
DOI:10.1684/ecn.2017.0395