The study of a fluorescent biosensor based on polyelectrolyte microcapsules with encapsulated glucose oxidase

A fluorescent biosensor is synthesized and described. The biosensor consists of polyelectrolyte microcapsules with glucose oxidase (GOx) entrapped in the cavities and an oxygen-sensitive fluorescent indicator Ru(dpp) immobilized in shells, where Ru(dpp) is tris(4,7-diphenyl-1,10-phenanthroline)ruthe...

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Bibliographic Details
Published in:Russian Journal of Physical Chemistry A 2017-09, Vol.91 (9), p.1828-1832
Main Authors: Kazakova, L. I., Sirota, N. P., Sirota, T. V., Shabarchina, L. I.
Format: Article
Language:English
Subjects:
Biophysical Chemistry
Biosensors
Chemistry
Chemistry and Materials Science
Composition effects
Fluorescence
Glucose oxidase
In vitro methods and tests
Microencapsulation
Physical Chemistry
Polyelectrolytes
Ruthenium
Ruthenium compounds
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Summary:A fluorescent biosensor is synthesized and described. The biosensor consists of polyelectrolyte microcapsules with glucose oxidase (GOx) entrapped in the cavities and an oxygen-sensitive fluorescent indicator Ru(dpp) immobilized in shells, where Ru(dpp) is tris(4,7-diphenyl-1,10-phenanthroline)ruthenium(II) dichloride. The theoretical activity of the encapsulated GOx and the effect storage time and medium composition have on the stability of sensor microcapsules are determined from polarographic measurements. No change in the activity of the encapsulated enzyme and or its loss to the storage medium are detected over the test period. The dispersion medium (water or a phosphate buffer) are shown to have no effect on the activity of microcapsules with immobilized GOx. The described optical sensor could be used as an alternative to electrochemical sensors for in vitro determination of glucose in the clinically important range of concentrations (up to 10 mmol/L).
ISSN:0036-0244
1531-863X
DOI:10.1134/S0036024417090151