Bioactive peptide from Pyropia yezoensis and its anti-inflammatory activities

• Published in: International journal of molecular medicine 2015-12, Vol.36 (6), p.1701-1706
• Main Authors: LEE, HYUN-AH, KIM, IN-HYE, NAM, TAEK-JEONG
• Format: Article
• Language: English
• Subjects: Amino acids; anti-inflammation; bioactive peptide; Care and treatment; Cell growth; Cytokines; Dose-response relationship (Biochemistry); Health aspects; Inflammation; Kinases; Nitric oxide; Peptides; Pharmacology, Experimental; Phosphorylation; Proteins; Pyropia yezoensis; RAW 264.7; Reactive oxygen species; Red algae; Rodents; Studies; Tumor necrosis factor-TNF
• ISSN: 1107-3756; 1791-244X
• DOI: 10.3892/ijmm.2015.2386

Pyropia yezoensis (P. yezoensis) is an important marine algae. Its high protein content serves as a good source of biologically active peptides. Potent inhibitory effects on the production of inflammatory mediators were observed in a bioactive peptide derived from P. yezoensis (peptide from P. yezoensis; PPY1), as demonstrated in lipopolysaccha-ride (LPS)-stimulated macrophages. The present study showed that peptide concentrations ranging from 250 to 1,000 ng/ml had no significant cytotoxicity in the cell viability assay when applied to the RAW 264.7 cells for 24 h. PPY1 completely inhibited LPS-stimulated nitric oxide (NO) release in a dose-dependent manner. Fluorescence intensity, corresponding to intracellular reactive oxygen species (ROS) produced by 10 ng/ml LPS-stimulated cells, significantly shifted, indicating that the peptide reduced the level of ROS. Furthermore, PPY1 exerted potent inhibitory activity to reduce the release of pro-inflammatory cytokines (inducible NO synthase, cyclo-oxygenase-2, interleukin-1β and tumor necrosis factor-α) in LPS-stimulated macrophages in a dose-dependent manner. These results also showed that the anti-inflammatory activity of PPY1 was associated with downregulation of extracellular signal-regulated kinase, protein 38, and c-jun NH2-terminal kinase phosphorylation in the mitogen-activated protein kinase pathways. In conclusion, PPY1 can have a significant role as an anti-inflammatory agent, with a potential for use in marine products.