LSm1 binds to the Dengue virus RNA 3′ UTR and is a positive regulator of Dengue virus replication

Dengue virus (DENV) is a mosquito-transmitted flavivirus that can cause severe disease in humans. The DENV positive strand RNA genome contains 5′ and 3′ untranslated regions (UTRs) that have been shown to be required for virus replication and interaction with host cell proteins. In the present study...

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Bibliographic Details
Published in:International journal of molecular medicine 2015-06, Vol.35 (6), p.1683-1689
Main Authors: YANGCHAO, DONG, JING, YANG, WEI, YE, YUAN, WANG, YUNBO, MIAO, TIANBING, DING, CHEN, XIANG, YINGFENG, LEI, ZHIKAI, XU
Format: Article
Language:English
Subjects:
Analysis
Cellular proteins
Dengue fever
Dengue virus
Dengue viruses
Disease transmission
Gene expression
Genomes
Genomics
host protein
Infections
LSm1
Microscopy
processing body
Proteins
RNA
Viral infections
viral replication
Viruses
West Nile virus
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Summary:Dengue virus (DENV) is a mosquito-transmitted flavivirus that can cause severe disease in humans. The DENV positive strand RNA genome contains 5′ and 3′ untranslated regions (UTRs) that have been shown to be required for virus replication and interaction with host cell proteins. In the present study LSm1 was identified as a host cellular protein involved in DENV RNA replication. By using two independent methodologies, we demonstrated a critical interaction between LSm1 and the 3′ UTR of DENV. Furthermore, the confocal immunofluorescence analysis showed that the interaction between LSm1 and viral RNA is located in P-body around nucleoli in the cytoplasm. LSm1 knockdown by siRNA specifically reduced the levels of viral RNA in DENV-infected cells and infectious DENV particles in the supernatant. These results provide evidence that LSm1 binding to the DENV RNA 3′ UTR positively regulates DENV RNA replication.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.2015.2169