Optimization of high pressure bioactive compounds extraction from pansies (Viola × wittrockiana) by response surface methodology

Response surface methodology (RSM) was employed for the first time to optimize high pressure extraction (HPE) conditions of bioactive compounds from pansies, namely: pressure (X 1 : 0-500 MPa), time (X 2 : 5-15 min) and ethanol concentration (X 3 : 0-100%). Consistent fittings using second-order pol...

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Bibliographic Details
Published in:High pressure research 2017-07, Vol.37 (3), p.415-429
Main Authors: Fernandes, Luana, Casal, Susana I.P., Pereira, José A., Ramalhosa, Elsa, Saraiva, Jorge A.
Format: Article
Language:English
Subjects:
Anthocyanins
antioxidant activity
bioactive compounds
Biocompatibility
Ethanol
Extraction
Extraction processes
Flavonoids
high pressure extraction
Optimization
Pansies flowers
Response surface methodology
Scavenging
Solvents
Tannins
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Summary:Response surface methodology (RSM) was employed for the first time to optimize high pressure extraction (HPE) conditions of bioactive compounds from pansies, namely: pressure (X 1 : 0-500 MPa), time (X 2 : 5-15 min) and ethanol concentration (X 3 : 0-100%). Consistent fittings using second-order polynomial models were obtained for flavonoids, tannins, anthocyanins, total reducing capacity (TRC) and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging activity. The optimum extraction conditions based on combination responses for TRC, tannins and anthocyanins were: X 1  = 384 MPa, X 2  = 15 min and X 3  = 35% (v/v) ethanol, shortening the extraction time when compared to the classic method of stirring (approx. 24 h). When the optimum extraction conditions were applied, 65.1 mg of TRC, 42.8 mg of tannins and 56.15 mg of anthocyanins/g dried flower were obtained. Thus, HPE has shown to be a promising technique to extract bioactive compounds from pansies, by reducing the extraction time and by using green solvents (ethanol and water), for application in diverse industrial fields.
ISSN:0895-7959
1477-2299
DOI:10.1080/08957959.2017.1347925