Sucrose hydrolysis by invertase immobilized on Duolite A-568 employing a packed-bed reactor

The conversion of sucrose to a highly concentrated commercial syrup by immobilized invertase by combining the processes of adsorption and cross-linking using Duolite A-568 as the carrier was studied. Central Composite Design (CCD) was used to assess the effect of glutaraldehyde concentration and cro...

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Bibliographic Details
Published in:Chemical engineering communications 2017-09, Vol.204 (9), p.1007-1019
Main Authors: Cabral, Bruna Vieira, Santos, Líbia Diniz, Santana Falleiros, Larissa N. S., Carmo, Taciana S., Freitas, Fernanda Ferreira, Cardoso, Saulo Luiz, Resende, Miriam M., Ribeiro, Eloízio Júlio
Format: Article
Language:English
Subjects:
Adsorption
Biocatalysts
Crosslinking
Enzyme activity
Glutaraldehyde
Hydrolysis
immobilization
Invertase
Reaction time
resin Duolite A-568
Sucrose
Syrup
Thermal stability
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Summary:The conversion of sucrose to a highly concentrated commercial syrup by immobilized invertase by combining the processes of adsorption and cross-linking using Duolite A-568 as the carrier was studied. Central Composite Design (CCD) was used to assess the effect of glutaraldehyde concentration and cross-linking reaction time on immobilized enzyme activity throughout the hydrolysis of sucrose in a batch reactor. Cross-linking optimization allowed us to find the optimum conditions for activity with a glutaraldehyde concentration of 0.6 g · L −1 and a cross-linking time of 6 h. The temperature and pH that maximized the activity of the immobilized biocatalyst in the cross-linking process were 50°C and 4.0, respectively. Cross-linking allows the biocatalyst to be active at higher temperatures and lower pH. High-sucrose conversions to invert sugar using a continuous fixed-bed reactor were obtained. The immobilized biocatalyst also demonstrated greater thermal stability at low temperatures.
ISSN:0098-6445
1563-5201
DOI:10.1080/00986445.2017.1336089