Detection of human neuronal [alpha]7 nicotinic acetylcholine receptors by conjugates of snake [alpha]-neurotoxin with quantum dots

Fluorescent derivatives are widely used to study the structure and functions of proteins. Quantum dots (QDs), fluorescent semiconductor nanocrystals, have a high quantum yield and are much more resistant to bleaching compared to organic dyes. Conjugates of [alpha]-neurotoxins with QDs were used for...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2017-07, Vol.475 (1), p.253
Main Authors: Makarova, Ya V, Shelukhina, I V, Mukherjee, A K, Kuznetsov, D V, Tsetlin, V I, Utkin, Yu N
Format: Article
Language:English
Subjects:
Acetylcholine
Acetylcholine receptors (nicotinic)
Adenoma
Bleaching
Conjugates
Nanocrystals
Neurology
Neurons
Neurotoxins
Pituitary
Proteins
Quantum dots
Snakes
Toxins
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Summary:Fluorescent derivatives are widely used to study the structure and functions of proteins. Quantum dots (QDs), fluorescent semiconductor nanocrystals, have a high quantum yield and are much more resistant to bleaching compared to organic dyes. Conjugates of [alpha]-neurotoxins with QDs were used for visualization of human [alpha]7 acetylcholine receptors heterologously expressed in GH4C1 pituitary adenoma cells. Specific staining of cells by the conjugated toxins was observed.
ISSN:1607-6729
1608-3091
DOI:10.1134/S1607672917040032