Development and Evaluation of a Loop-mediated Isothermal Amplification Combined with Au-nanoprobe Assay for Rapid Detection of Mycobacterium tuberculosis

Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 cl...

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Bibliographic Details
Published in:Indian journal of medical microbiology 2017-04, Vol.35 (2), p.302-304
Main Authors: Kaewphinit, Thongchai, Ckumdee, Jutturong, Chansiri, Kosum, Santiwatanakul, Somchai
Format: Article
Language:English
Subjects:
Assaying
Au-nanoprobe
Colorimetry
Deoxyribonucleic acid
diagnosis
DNA
Infectious diseases
Laboratories
loop-mediated isothermal amplification
Low cost
Microscopy
Mycobacterium tuberculosis
Polymerase chain reaction
Public health
Reagents
Researchers
Tuberculosis
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Summary:Loop-mediated isothermal amplification (LAMP) has been proposed as an inexpensive and easy to perform assay for molecular diagnostics. We present a novel strategy for the detection of LAMP amplicons derived from Mycobacterium tuberculosis by the use of Au-nanoprobes. When applied to a total of 93 clinical specimens, the LAMP assay demonstrated sensitivity and specificity higher than that of polymerase chain reaction and culture. The Au-nanoprobe augmented LAMP test platform with its advantages of robust reagents and a simple colorimetric detection method can be adapted easily for the rapid detection of other infectious disease agents at a low cost.
ISSN:0255-0857
1998-3646
DOI:10.4103/ijmm.IJMM_15_333