Levels of Circulating Immune Complexes Containing Mycobacterium Tuberculosis-specific Antigens in Pulmonary Tuberculosis and Sarcoidosis Patients

The present study was conducted to understand the aetiological link between tuberculosis (TB) and sarcoidosis. Sera from smear-positive TB subjects (n = 24), smear-negative TB subjects (n = 24), sarcoidosis patients (n = 24) and healthy controls (n = 24) were collected and circulating immune complex...

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Bibliographic Details
Published in:Indian journal of medical microbiology 2017-04, Vol.35 (2), p.290-292
Main Authors: Goyal, B., Sheikh, J.A., Agarwal, R., Verma, I.
Format: Article
Language:English
Subjects:
Antigen-antibody complexes
Antigens
Biochemistry
Circulating immune complexes
Deoxyribonucleic acid
DNA
Enzyme-linked immunosorbent assay
Filtrate
Health education
Immunoassay
Immunoassays
Immunoglobulins
Laboratories
Mathematical analysis
Patients
Peptides
Proteins
pulmonary tuberculosis
regions of differences antigens
sandwich ELISA
Sarcoidosis
Smear
Studies
Tuberculosis
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Summary:The present study was conducted to understand the aetiological link between tuberculosis (TB) and sarcoidosis. Sera from smear-positive TB subjects (n = 24), smear-negative TB subjects (n = 24), sarcoidosis patients (n = 24) and healthy controls (n = 24) were collected and circulating immune complexes were isolated. Sandwich ELISA was performed for detecting four highly specific mycobacterial regions of difference (RD) proteins (early secretory antigenic target 6 [ESAT6], 10 KDa culture filtrate protein [CFP10], 21 KDa CFP [CFP21] and mycobacterial protein from species TB [MPT 64]). Sensitivity and specificity was calculated, and receiver operating characteristic plots were plotted. Non-parametric Mann–Whitney U-test was used to calculate statistical significance. Seventy per cent of sarcoidosis patients showed the presence of immune complexes of mycobacterial RD proteins similar to that observed in the sera of smear-negative TB patients as opposed to antibody-based detection assay based on these RD proteins. Thus, immunoassays based on specific mycobacterial RD proteins also need to be developed and validated carefully to differentiate TB and sarcoidosis, a close mimic of smear-negative tuberculosis.
ISSN:0255-0857
1998-3646
DOI:10.4103/0255-0857.209569