Quantitative analysis and QTL mapping for agronomic and fiber traits in an RI population of upland cotton

• Published in: Euphytica 2009-01, Vol.165 (2), p.231-245
• Main Authors: Wu, Jixiang, Gutierrez, Osman Ariel, Jenkins, Johnie N, McCarty, Jack C, Zhu, Jun
• Format: Article
• Language: English
• Subjects: agronomic traits; Agronomy; Agronomy. Soil science and plant productions; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; chromosome mapping; Chromosomes; Cotton; crop yield; fiber quality; fiber traits; Fundamental and applied biological sciences. Psychology; Gene mapping; Genetic linkage; genetic markers; Genetics and breeding of economic plants; Genomics; Gossypium hirsutum; inbred lines; Inbreeding; Life Sciences; linkage groups; lint cotton; loci; microsatellite repeats; phenotypic variation; Phenotypic variations; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; quantitative genetics; quantitative trait loci; quantitative traits; recombinant inbred lines
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-008-9748-8

Genetic mapping is an essential tool for cotton (Gossypium hirsutum L.) molecular breeding and application of DNA markers for cotton improvement. In this present study, we evaluated an RI population including 188 RI lines developed from 94 F₂-derived families and their two parental lines, 'HS 46' and 'MARCABUCAG8US-1-88', at Mississippi State, MS, for two years. Fourteen agronomic and fiber traits were measured. One hundred forty one (141) polymorphic SSR markers were screened for this population and 125 markers were used to construct a linkage map. Twenty six linkage groups were constructed, covering 125 SSR loci and 965 cM of overall map distance. Twenty four linkage groups (115 SSR loci) were assigned to specific chromosomes. Quantitative genetic analysis showed that the genotypic effects accounted for more than 20% of the phenotypic variation for all traits except fiber perimeter (18%). Fifty six QTLs (LOD > 3.0) associated with 14 agronomic and fiber traits were located on 17 chromosomes. One QTL associated with fiber elongation was located on linkage group LGU01. Nine chromosomes in sub-A genome harbored 27 QTLs with 10 associated with agronomic traits and 17 with fiber traits. Eight chromosomes in D sub-genome harbored 29 QTLs with 13 associated with agronomic traits and 16 with fiber traits. Chromosomes 3, 5, 12, 13, 14, 16, 20, and 26 harbor important QTLs for both yield and fiber quality compared to other chromosomes. Since this RI population was developed from an intraspecific cross within upland cotton, these QTLs should be useful for marker assisted selection for improving breeding efficiency in cotton line development.