Short Communication - PCR-Based Detection of a Hyperparasitoid in Cotton Mealybug, Phenacoccus solenopsis Tinsley

PCR protocol was developed to detect hyperparasitism in cotton mealybug. Cotton mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae) is an invasive and major pest on cotton. Aenasius bambawalei (Hymenoptera: Encyrtidae) is an effective parasitoid of mealybug and has been the backbone of the...

Full description

Saved in:
Bibliographic Details
Published in:Pakistan journal of zoology 2017-08, Vol.49 (4), p.1515
Main Authors: Ashfaq, Muhammad, Akhtar, Shamim, Akhtar, Saleem, Masood, Mariyam
Format: Article
Language:English
Subjects:
Aenasius bambawalei
Biological control
Biological pest control
Control
Cotton
Cytochrome
Cytochrome oxidase I
Cytochromes
Deoxyribonucleic acid
DNA
Gene sequencing
Genotypes
Hemiptera
Hymenoptera
Hyperparasitism
Identification and classification
Innovations
Mealybugs
Mitochondrial DNA
Morphology
Nucleotide sequence
Parasitoids
Phenacoccus solenopsis
Polymerase chain reaction
Population
Promuscidea unfasciativentris
Pseudococcidae
Studies
Taxonomy
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:PCR protocol was developed to detect hyperparasitism in cotton mealybug. Cotton mealybug, Phenacoccus solenopsis (Hemiptera: Pseudococcidae) is an invasive and major pest on cotton. Aenasius bambawalei (Hymenoptera: Encyrtidae) is an effective parasitoid of mealybug and has been the backbone of the biological control program of cotton mealybug in Pakistan, but its multiplication for inundative releases has been impacted by a hyperparasitoid, Promuscidea unfasciativentris Girault (Hymenoptera: Aphelinidae: Eriaporinae). Traditionally the detection of hyperparasitoid is done by host-rearing, but that is time-consuming when working with field populations at larger scale.The current study used morphology and DNA sequences from mitochondrial (cytochrome oxidase I) and nuclear (internal transcribed spacer I) genes to identify the hyperparasitoid Promuscidea unfasciativentris. The successful PCR-based detection of hyperparasitism is promising for designing strategies for the biological control program of cotton mealybug.
ISSN:0030-9923